Moorby C D, Stoker M, Gherardi E
Imperial Cancer Research Fund, Cambridge University Medical School, United Kingdom.
Exp Cell Res. 1995 Aug;219(2):657-63. doi: 10.1006/excr.1995.1276.
Hepatocyte growth factor/scatter factor (HGF/SF) is a fibroblast-derived protein that affects the growth, motility, and differentiation of epithelial and endothelial cells. We have investigated the effect of HGF/SF on junctional communication in mouse keratinocytes (MK cells). HGF/SF inhibited cell communication in MK cells as assessed by the transfer of a low-molecular-weight dye, Lucifer Yellow. The inhibition was rapid, the earliest effects being apparent 5 to 10 min after addition of the factor, and was transient. The decrease in dye transfer correlated with a loss of the gap junction protein connexin 43 as measured by Western blotting, probably due to increased protein degradation. The results show that junctional communication is an early target of HGF/SF activity and they are consistent with the hypothesis that gap junctions are primary targets of the action of growth factors.
肝细胞生长因子/散射因子（HGF/SF）是一种由成纤维细胞产生的蛋白质，它影响上皮细胞和内皮细胞的生长、运动及分化。我们研究了HGF/SF对小鼠角质形成细胞（MK细胞）中连接通讯的影响。通过低分子量染料荧光素黄的转移评估，HGF/SF抑制了MK细胞中的细胞通讯。这种抑制作用迅速，在添加该因子后最早5到10分钟就明显出现，并且是短暂的。通过蛋白质印迹法检测，染料转移的减少与缝隙连接蛋白连接蛋白43的丢失相关，这可能是由于蛋白质降解增加所致。结果表明，连接通讯是HGF/SF活性的早期靶点，并且与缝隙连接是生长因子作用的主要靶点这一假说相符。
