An attempt at the use of immunological methods for the detection of enzymes of BCG substrains.

Immunological methods have been used to visualize peroxidase and dehydrogenase activities of substrains of BCG mycobacteria. Immunodiffusion (ID) and immunoelectrophoresis (IE) in agar gel and two-dimensional immunoelectrophoresis (2D-IE) in agarose gel were employed. In all these tests part of the ID, IE, and 2D-IE preparations were stained with Amido Black 10B (AB 10B) to visualize the total number of precipitin lines and part were stained at the same time with histochemical methods for vizualization of peroxidase and dehydrogenase activities.