Ohman R, Ridell M
Department of Medical Microbiology and Immunology, University of Göteborg, Sweden.
Curr Microbiol. 1995 Mar;30(3):161-5. doi: 10.1007/BF00296202.
Strains of Mycobacterium bovis, M. bovis BCG, and M. tuberculosis, including a so-called Canetti strain, were analyzed by means of two-dimensional immunoelectrophoresis (2D-IE), 2D-IE combined with enzyme staining, and multilocus enzyme electrophoresis (MEE). The results demonstrated a close antigenic and enzymatic resemblance among all the strains tested, even though the BCG strains could be divided into two groups based on the presence of one precipitinogen. Eight of the precipitinogens were shown to correspond to enzymes in M. bovis BCG and 10 in M. tuberculosis. Thus, catalase, isocitrate dehydrogenase, malate dehydrogenase, peroxidase, and several others were identified. By means of MEE the strains of M. tuberculosis, M. bovis, and M. bovis BCG could be differentiated. The analyses further indicated that the M. tuberculosis strain Canetti was more closely related to M. bovis than to M. tuberculosis.
利用二维免疫电泳（2D - IE）、二维免疫电泳结合酶染色以及多位点酶电泳（MEE）对牛分枝杆菌、卡介苗分枝杆菌以及结核分枝杆菌菌株，包括一种所谓的卡内蒂菌株进行了分析。结果表明，尽管卡介苗菌株可根据一种沉淀原的存在分为两组，但所有测试菌株之间在抗原和酶方面存在密切的相似性。其中八种沉淀原被证明对应于卡介苗分枝杆菌中的酶，十种对应于结核分枝杆菌中的酶。因此，过氧化氢酶、异柠檬酸脱氢酶、苹果酸脱氢酶、过氧化物酶等得以鉴定。通过MEE可以区分结核分枝杆菌、牛分枝杆菌和卡介苗分枝杆菌的菌株。分析进一步表明，结核分枝杆菌卡内蒂菌株与牛分枝杆菌的关系比与结核分枝杆菌的关系更为密切。
