Hildebrandt H, Müller U
Institut für Neurobiologie, Freien Universität Berlin, Germany.
J Neurobiol. 1995 May;27(1):44-50. doi: 10.1002/neu.480270105.
In the honeybee octopamine mediates mechanisms of arousal that interfere with the appetitive proboscis extension response to food-indicating chemosensory stimuli. This study demonstrates that injections of octopamine or cyclic adenosine monophosphate (cAMP) into the primary chemosensory neuropil of the honeybee, the antennal lobe, evokes a rapid and transient activation of cAMP-dependent protein kinase (PKA). Other monoamines detectable in the antennal lobe, dopamine and serotonin, do not affect the level of PKA activity. Stimulation of the bees' antenna with the appetitive stimulus water or sucrose solution in vivo also causes a short-term activation of PKA in the antennal lobe. The increased PKA activity can be detected immediately (0.5 s) after stimulation but reverts to the basal level within 3 s. This effect can be abolished by monoamine depletion with reserpine. Since octopamine is the only monoamine that stimulates PKA, it appears to mediate the PKA activation after sucrose stimulus and may contribute to the processing of this chemosensory input.
在蜜蜂中，章鱼胺介导唤醒机制，该机制会干扰对指示食物的化学感官刺激的食欲性喙伸展反应。本研究表明，向蜜蜂的主要化学感觉神经纤维束即触角叶注射章鱼胺或环磷酸腺苷（cAMP），会引发cAMP依赖性蛋白激酶（PKA）的快速短暂激活。在触角叶中可检测到的其他单胺，多巴胺和血清素，不会影响PKA活性水平。用食欲性刺激物水或蔗糖溶液在体内刺激蜜蜂的触角，也会导致触角叶中PKA的短期激活。刺激后立即（0.5秒）即可检测到PKA活性增加，但在3秒内恢复到基础水平。用利血平进行单胺耗竭可消除这种效应。由于章鱼胺是唯一能刺激PKA的单胺，它似乎在蔗糖刺激后介导PKA激活，并可能有助于这种化学感觉输入的处理。
