Fukuse T, Albes J M, Takahashi Y, Brandes H, Hausen B, Schäfers H J
Department of Thoracic Surgery, Kyoto University, Japan.
J Surg Res. 1995 Sep;59(3):399-404. doi: 10.1006/jsre.1995.1182.
Crystalloid perfusates commonly are utilized for lung preservation in extracorporeal small animal lung models. However, the function of these grafts is limited. In a new ex-vivo rat heart-lung model the role of red blood cells added to the crystalloid perfusate was investigated. Heart-lung blocks were rapidly excised (n = 9, each group) and the blocks were connected to the extracorporeal perfusion circuit using Krebs-Henseleit solution (KH) or KH with washed bovine red blood cells (hematocrit 38%) (KHRBC). The lungs were ventilated with room air. The coronary system was perfused via the aortic root with oxygenated perfusate. The lungs were perfused via the right ventricle with deoxygenated perfusate (PO2 15 mm Hg). Venoarterial improvement of oxygenation, pulmonary vascular resistance (PVR), and peak inspiratory pressure (PIP) were continuously monitored for 50 min. At the end of the experiment the wet/dry (W/D) ratio was determined using the mediastinal lung lobe while the remaining lung was used for light microscopic (LM) evaluation. After 30 min of perfusion, lung function was significantly better in the KHRBC group (PVR (KH): 752 +/- 193 dynseccm-5; (KHRBC): 279 +/- 48 dynseccm-5; PIP (KH): 31 +/- 3.2 mm Hg; (KHRBC): 25.8 +/- 1.9 mm Hg). In addition, lungs perfused with KHRBC showed significantly less edema than those perfused with KH only (W/D ratio (KH): 7.8 +/- 1.2; (KHRBC) 5.1 +/- 0.6; LM (KH): 3.5 +/- 0.9; (KHRBC): 2.3 +/- 0.8). The use of red blood cells in KH perfusate improved functional and structural integrity.(ABSTRACT TRUNCATED AT 250 WORDS)
晶体灌注液常用于体外小动物肺模型的肺保存。然而，这些移植肺的功能有限。在一个新的离体大鼠心肺模型中，研究了添加到晶体灌注液中的红细胞的作用。迅速切除心肺块（每组n = 9），并使用克雷布斯 - 亨泽莱特溶液（KH）或含洗涤过的牛红细胞（血细胞比容38%）的KH（KHRBC）将心肺块连接到体外灌注回路。用室内空气对肺进行通气。通过主动脉根部用含氧灌注液灌注冠状动脉系统。通过右心室用脱氧灌注液（PO2 15 mmHg）灌注肺。连续监测50分钟的静脉动脉氧合改善、肺血管阻力（PVR）和吸气峰压（PIP）。实验结束时，使用纵隔肺叶测定湿/干（W/D）比，而其余肺用于光学显微镜（LM）评估。灌注30分钟后，KHRBC组的肺功能明显更好（PVR（KH）：752±193 dynseccm-5；（KHRBC）：279±48 dynseccm-5；PIP（KH）：31±3.2 mmHg；（KHRBC）：25.8±1.9 mmHg）。此外，与仅用KH灌注的肺相比，用KHRBC灌注的肺水肿明显更少（W/D比（KH）：7.8±1.2；（KHRBC）5.1±0.6；LM（KH）：3.5±0.9；（KHRBC）：2.3±0.8）。在KH灌注液中使用红细胞改善了功能和结构完整性。（摘要截断于250字）
