胎儿脑移植对新生期雄激素化雌性大鼠成年行为雄性化和雌性化消除的影响。

Effects of fetal brain grafting on adult behavioral masculinization and defeminization in neonatally androgenized female rats.

作者信息

Tsai Y F, Chen T J, Pi W P, Tai M Y, Huang R L, Chiueh C C, Peng M T

机构信息

Department of Physiology, College of Medicine, National Taiwan University, Taipei, Republic of China.

出版信息

Neurosci Lett. 1995 May 5;190(2):97-100. doi: 10.1016/0304-3940(95)11510-4.

DOI:10.1016/0304-3940(95)11510-4

PMID:7644131

Abstract

Treatment of neonatal female rats with androgen results not only in decreased female sexual behavior but also in enhanced male sexual behavior examined in adulthood. The effects of grafting fetal preoptic area (POA) neurons into the POA, and fetal hypothalamic (HPT) neurons into the ventromedial hypothalamus (VMH), were tested in neonatally androgen-sterilized rats (ASR). The rats were injected subcutaneously with 80 micrograms testosterone propionate within the 24 hours after birth to see if sexual behavior could be normalized by fetal brain grafts. In repeated tests on ASR grafted with fetal HPT into the VMH, the lordotic response was seen to increase to the level seen in non-ASR controls, while the increase in mounting behavior in ASR was suppressed following grafting of fetal POA or cerebral cortex into the POA. These results suggest that there are dysfunctions of POA and VMH in ASR, and that the dysfunctions revealed by sexual behavior can be overcome by fetal POA or HPT grafting.

摘要

用雄激素处理新生雌性大鼠，不仅会导致成年后雌性性行为减少，还会增强雄性性行为。在新生期经雄激素绝育的大鼠（ASR）中，测试了将胎儿视前区（POA）神经元移植到POA以及将胎儿下丘脑（HPT）神经元移植到腹内侧下丘脑（VMH）的效果。在出生后24小时内，给这些大鼠皮下注射80微克丙酸睾酮，以观察胎儿脑移植是否能使性行为恢复正常。在对将胎儿HPT移植到VMH的ASR进行的重复测试中，发现脊柱前凸反应增加到了非ASR对照中的水平，而在将胎儿POA或大脑皮层移植到POA后，ASR中骑跨行为的增加受到了抑制。这些结果表明，ASR中POA和VMH存在功能障碍，并且性行为所揭示的功能障碍可以通过胎儿POA或HPT移植来克服。