Flamant F, Samarut J
Laboratoire de Biologie INRA-CNRS Ecole Normale Supérieure de Lyon, France.
Virology. 1995 Aug 1;211(1):234-40. doi: 10.1006/viro.1995.1396.
Virofection is a simple new way to use replication-defective vectors. It consists of the cotransfection of two plasmids: one plasmid bearing the genome of the replication-defective retrovirus vector and a second "helper" plasmid expressing the gag, pol, and env retrovirus sequences. It provides stable integration into the chromosome and long-term expression of only vector-borne sequences. We present here several helper plasmids derived from avian leukosis viruses, which we have used for the virofection of the lacZ reporter gene in chicken cell cultures. Expression can be stabilized at a very high rate in both chicken fibroblasts and blastoderm cells, without giving rise to replication-competent virus.
病毒感染是一种使用复制缺陷型载体的简单新方法。它由两种质粒的共转染组成：一种质粒携带复制缺陷型逆转录病毒载体的基因组，另一种“辅助”质粒表达gag、pol和env逆转录病毒序列。它能稳定整合到染色体中，并且仅载体携带的序列能长期表达。我们在此展示了几种源自禽白血病病毒的辅助质粒，我们已将其用于鸡细胞培养物中lacZ报告基因的病毒感染。在鸡成纤维细胞和胚盘细胞中，表达都能以非常高的比率稳定下来，且不会产生具有复制能力的病毒。
