Winter J, Hall R L, Moyer R W
Department of Molecular Genetics and Microbiology, University of Florida, College of Medicine, Gainesville 32610-0266, USA.
Virology. 1995 Aug 20;211(2):462-73. doi: 10.1006/viro.1995.1428.
Within the family of Poxviridae, the entomopoxviruses are the most distant relatives of the more well-known and intensively studied orthopoxviruses (vaccinia and variola). The growth of the entomopoxvirus from Amsacta moorei (AmEPV) has been characterized in cell culture and compared to that of vaccinia virus (VV), the prototypic orthopoxvirus. The overall characteristics of infected cell cultures were generally similar between the two viruses. One striking difference noted was the apparent absence of proteolytic processing of late AmEPV viral proteins, a hallmark of vertebrate poxvirus infections associated with viral morphogenesis. AmEPV, like VV, was found to be sensitive to all the inhibitors of viral infection tested including phosphonoacetic acid, 1-beta-D-arabinofuranosylcytosine, and isatin-beta-thiosemicarbazone (IBT), a compound associated with the rather specific inhibition of vertebrate poxviruses. While both VV and AmEPV are inhibited by IBT, the inhibition of AmEPV, unlike that of VV, is not accompanied by either a breakdown of ribosomal RNA or a global inhibition of late viral protein synthesis. Instead, in the presence of IBT, AmEPV enveloped, immature virions form devoid of a well-differentiated core, which unlike mature virions fail to insert into occlusion bodies.
在痘病毒科中，昆虫痘病毒是更为知名且深入研究的正痘病毒（痘苗病毒和天花病毒）的最远亲族。来自摩尔按实蝇的昆虫痘病毒（AmEPV）的生长特性已在细胞培养中得到表征，并与正痘病毒的原型——痘苗病毒（VV）进行了比较。两种病毒感染细胞培养物的总体特征通常相似。所观察到的一个显著差异是，晚期AmEPV病毒蛋白明显缺乏蛋白水解加工，而这是与病毒形态发生相关的脊椎动物痘病毒感染的一个标志。与VV一样，发现AmEPV对所有测试的病毒感染抑制剂敏感，包括膦甲酸、1-β-D-阿拉伯呋喃糖基胞嘧啶和异烟肼-β-硫代半卡巴腙（IBT），后者是一种与对脊椎动物痘病毒有相当特异性抑制作用相关的化合物。虽然VV和AmEPV都受到IBT的抑制，但与VV不同的是，AmEPV的抑制并未伴随着核糖体RNA的降解或晚期病毒蛋白合成的全面抑制。相反，在存在IBT的情况下，AmEPV包膜的未成熟病毒粒子形成，缺乏分化良好的核心，与成熟病毒粒子不同的是，它们无法插入包涵体。
