Rauk P N, Surti U, Roberts J M, Michalopoulos G
Department of Obstetrics, Gynecology, and Reproductive Science, University of Pittsburgh, PA, USA.
Am J Obstet Gynecol. 1995 Aug;173(2):571-7. doi: 10.1016/0002-9378(95)90284-8.
We compared the mitogenic effect of basic fibroblast growth factor with and without estradiol on myometrial and leiomyometrial cells.
The mitogenic effect of basic fibroblast growth factor on myometrial cells was measured by thymidine incorporation and cell count. The mitogenic effect of basic fibroblast growth factor with and without estradiol as measured by thymidine incorporation was compared between myometrial and leiomyometrial cells.
Both human myometrial and leiomyometrial cells showed significant (p = 0.004 and p = 0.001, respectively), dose-dependent incorporation of thymidine in response to basic fibroblast growth factor. Leiomyometrial cells showed significantly (p = 0.04) less thymidine incorporation compared with matched normal myometrial cells. The addition of estradiol with basic fibroblast growth factor did not result in a further increase in thymidine incorporation.
Both myometrial and leiomyometrial cells respond to basic fibroblast growth factor with increased thymidine incorporation; however leiomyometrial cells are less responsive than are matched normal myometrial cells. The addition of estradiol is not synergistic with basic fibroblast growth factor.
我们比较了有或没有雌二醇时碱性成纤维细胞生长因子对子宫肌层细胞和平滑肌-肌层细胞的促有丝分裂作用。
通过胸腺嘧啶核苷掺入法和细胞计数来测量碱性成纤维细胞生长因子对子宫肌层细胞的促有丝分裂作用。比较了子宫肌层细胞和平滑肌-肌层细胞中，有或没有雌二醇时碱性成纤维细胞生长因子通过胸腺嘧啶核苷掺入法测得的促有丝分裂作用。
人子宫肌层细胞和平滑肌-肌层细胞对碱性成纤维细胞生长因子均有显著（分别为p = 0.004和p = 0.001）的剂量依赖性胸腺嘧啶核苷掺入。与配对的正常子宫肌层细胞相比，平滑肌-肌层细胞的胸腺嘧啶核苷掺入显著减少（p = 0.04）。碱性成纤维细胞生长因子与雌二醇一起添加并未导致胸腺嘧啶核苷掺入进一步增加。
子宫肌层细胞和平滑肌-肌层细胞对碱性成纤维细胞生长因子的反应均是胸腺嘧啶核苷掺入增加；然而，平滑肌-肌层细胞的反应性低于配对的正常子宫肌层细胞。添加雌二醇与碱性成纤维细胞生长因子没有协同作用。
