Pasqualini J R, Chen T A, Maloche C, Chetrite G, Valentin E, Allfrey V
C.N.R.S. Steroid Hormone Research Unit, Foundation for Hormone Research, Paris, France.
Anticancer Res. 1995 May-Jun;15(3):671-4.
The effects of the conditioned medium (CM) of embryonic mouse fibroblast (BALB/c-3T3) cells (clone A31) on the mRNA of the c-myc oncogene of the hormone-dependent T-47D mammary cancer cells were explored. After 6h incubation a significant stimulation of c-myc expression was observed with a 10% v/v concentration of CM, and the effect was even higher using a CM concentration of 30% v/v. It is suggested that this action of CM could be related to the stimulatory effect on proliferation and DNA synthesis induced by the CM, as was recently demonstrated for the T-47D cells. It was also observed that the antiestrogen ICI 164,384 (5 x 10(-6) M) can inhibit the stimulatory action of CM on c-myc expression. It is concluded that the fibroblast mouse embryonic cells contain and secrete factor(s) involved in the mechanism of proliferation of human hormone-dependent mammary cancer cells.
探讨了胚胎小鼠成纤维细胞（BALB/c-3T3细胞，克隆A31）的条件培养基（CM）对激素依赖性T-47D乳腺癌细胞c-myc癌基因mRNA的影响。孵育6小时后，在CM浓度为10%（v/v）时观察到c-myc表达有显著刺激，而使用30%（v/v）的CM浓度时效果更高。提示CM的这种作用可能与CM诱导的对增殖和DNA合成的刺激作用有关，正如最近在T-47D细胞中所证实的那样。还观察到抗雌激素ICI 164,384（5×10⁻⁶ M）可抑制CM对c-myc表达的刺激作用。得出的结论是，小鼠胚胎成纤维细胞含有并分泌参与人类激素依赖性乳腺癌细胞增殖机制的因子。
