Buszello H
Department of Urology, Rheinische Friedrich-Wilhelms-Universität, Bonn, Germany.
Anticancer Res. 1995 May-Jun;15(3):735-8.
The effect of alpha-Interferon (alpha-IFN), gamma-interferon (gamma-IFN), tumor necrosis factor alpha (TNF-alpha) and interleukin-2 (Il-2) on growth of two renal cancer cell lines (DNT-11, HTB-44) was studied. Cell proliferation was measured by a tetrazolium based microculture assay (MTT) after incubation of tumor cells with different concentrations of these 4 cytokines over 72 hours. TNF-alpha showed no antiproliferative effect when cell line DNT-11 was tested, even at high concentrations of 1.000 ng/ml. Proliferation of the other cell line (HTB-44) was only slightly reduced (15%) at 100 ng/ml; a tenfold increase of TNF concentration could not improve these results. The antiproliferative effect of alpha-IFN was in the range of 22-26%. When gamma-IFN was used, both cell lines showed reduction of proliferation between 26% and 37%, respectively. Kinetic studies revealed that prolongation of incubation time for more than 72 hours was not able to improve these results. Il-2 did not influence cell proliferation of either cell line tested, even at high concentrations, as expected.
研究了α-干扰素（α-IFN）、γ-干扰素（γ-IFN）、肿瘤坏死因子α（TNF-α）和白细胞介素-2（IL-2）对两种肾癌细胞系（DNT-11、HTB-44）生长的影响。在肿瘤细胞与不同浓度的这4种细胞因子孵育72小时后，通过基于四氮唑的微量培养法（MTT）测量细胞增殖。当检测细胞系DNT-11时，即使在1000 ng/ml的高浓度下，TNF-α也未显示出抗增殖作用。另一种细胞系（HTB-44）在100 ng/ml时增殖仅略有降低（15%）；TNF浓度增加十倍也未能改善这些结果。α-IFN的抗增殖作用在22%至26%之间。使用γ-IFN时，两种细胞系的增殖分别降低了26%至37%。动力学研究表明，将孵育时间延长至72小时以上并不能改善这些结果。如预期的那样，即使在高浓度下，IL-2也不影响所测试的任一细胞系的细胞增殖。
