Eberhagen I, Bankmann M, Kaina B
Research Center Karlsruhe, Department of Genetics, Karlsruhe, Germany.
Anticancer Res. 1995 May-Jun;15(3):761-7.
The DNA repair protein O6-methylguanine-DNA methyltransferase (MGMT) is a main determinant of resistance of tumor cells towards nitrosoureas that are in use as cytostatic drugs. It therefore appears of importance to determine its expression in tumors before the utilization of nitrosoureas for tumor treatment. We have determined the level of expression of MGMT in mammary carcinomas and brain tumors by means of in situ hybridization, using digoxygenin-labeled RNA driven by MGMT expression vector in sense and antisense orientation. It is shown that the intensity of the hybridization signal correlates with the MGMT activity of a given tumor. The results demonstrate the applicability of in situ hybridization for determination of MGMT expression in tumor sections.
DNA修复蛋白O6-甲基鸟嘌呤-DNA甲基转移酶（MGMT）是肿瘤细胞对用作细胞抑制药物的亚硝基脲产生耐药性的主要决定因素。因此，在使用亚硝基脲进行肿瘤治疗之前，确定其在肿瘤中的表达似乎很重要。我们通过原位杂交，使用由MGMT表达载体以正义和反义方向驱动的地高辛标记RNA，测定了乳腺癌和脑肿瘤中MGMT的表达水平。结果表明，杂交信号的强度与给定肿瘤的MGMT活性相关。这些结果证明了原位杂交在测定肿瘤切片中MGMT表达方面的适用性。
