Sakagami H, Takeda M, Sugaya K, Omata T, Takahashi H, Yamamura M, Hara Y, Shimamura T
First Department of Biochemistry, School of Medicine, Showa University, Tokyo, Japan.
Anticancer Res. 1995 May-Jun;15(3):971-4.
(-) Epigallocatechin gallate (EGCg) potently stimulated the production of interleukin-1 (IL-1) and tumor necrosis factor by human peripheral blood mononuclear cells. The intracellular amounts of IL-1 beta and especially IL-1 alpha induced by EGCg, were significantly higher than their extracellular counterparts. ECCg stimulated the production of adherent cells, with IL-1 producing capacity (per cell basis) that was significantly higher than nonadherent cells. Although IL-1 alpha mRNA synthesis (assessed by Reverse Transcriptase-Polymerase Chain Reaction) was slightly enhanced, IL-1 beta mRNA synthesis was not significantly enhanced by EGCg treatment. Lipopolysaccharide (LPS) also stimulated the production of IL-1 alpha and IL-1 beta production, but failed to induce the adherent cells. These data suggest that EGCg and LPS stimulate mononuclear cells by different mechanisms.
（-）表没食子儿没食子酸酯（EGCg）可有效刺激人外周血单个核细胞产生白细胞介素-1（IL-1）和肿瘤坏死因子。EGCg诱导的细胞内IL-1β尤其是IL-1α的量显著高于其细胞外对应物。EGCg刺激具有IL-1产生能力（基于每个细胞）的贴壁细胞的产生，其产生能力显著高于非贴壁细胞。尽管通过逆转录聚合酶链反应评估的IL-1αmRNA合成略有增强，但EGCg处理并未显著增强IL-1βmRNA合成。脂多糖（LPS）也刺激IL-1α和IL-1β的产生，但未能诱导贴壁细胞。这些数据表明EGCg和LPS通过不同机制刺激单核细胞。
