Sakagami H, Sugaya K, Utsumi A, Fujinaga S, Sato T, Takeda M
First Department of Biochemistry, School of Medicine, Showa University, Tokyo, Japan.
Anticancer Res. 1993 May-Jun;13(3):671-5.
PSK (Krestin), a protein-bound polysaccharide extracted from Coriolus versicolor, stimulated the production of interleukin-1 (IL-1) by human peripheral blood mononuclear cells more efficiently than the production of tumor necrosis factor (TNF). More IL-1 alpha was accumulated in the cells than in the medium fraction, whereas IL-1 beta was distributed evenly into both fractions. PSK stimulated the production of adherent mononuclear cells, in which significantly higher amounts of IL-1 alpha/IL-1 beta were accumulated per cell than in nonadherent cells. Although IL-1 alpha mRNA synthesis (assessed by Reverse Transcriptase-Polymerase Chain Reaction) was slightly enhanced, IL-1 beta mRNA synthesis was not significantly changed by PSK treatment. This suggests that PSK might increase the efficiency of IL-1 mRNA translation or the posttranslational processing of IL-1 protein. Despite potent cytokine-inducing activity, lipopolysaccharide (LPS) did not significantly stimulate the production of adherent cells. These data suggest that PSK and LPS might stimulate mononuclear cells by different mechanisms.
PSK（云芝多糖K）是一种从云芝中提取的蛋白结合多糖，它刺激人外周血单个核细胞产生白细胞介素-1（IL-1）的效率比产生肿瘤坏死因子（TNF）的效率更高。细胞内积累的IL-1α比培养基部分更多，而IL-1β则均匀分布于两个部分。PSK刺激贴壁单核细胞的产生，其中每个细胞积累的IL-1α/IL-1β量显著高于非贴壁细胞。尽管PSK处理使IL-1α mRNA合成（通过逆转录-聚合酶链反应评估）略有增强，但IL-1β mRNA合成没有显著变化。这表明PSK可能会提高IL-1 mRNA翻译效率或IL-1蛋白的翻译后加工效率。尽管脂多糖（LPS）具有强大的细胞因子诱导活性，但它并没有显著刺激贴壁细胞的产生。这些数据表明，PSK和LPS可能通过不同机制刺激单核细胞。
