在反硝化副球菌完整细胞中鉴定周质硝酸还原酶Mo(V)的电子顺磁共振信号。
Identification of periplasmic nitrate reductase Mo(V) EPR signals in intact cells of Paracoccus denitrificans.
作者信息
Sears H J, Bennett B, Spiro S, Thomson A J, Richardson D J
机构信息
Centre for Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, Norwich, UK.
出版信息
Biochem J. 1995 Aug 15;310 ( Pt 1)(Pt 1):311-4. doi: 10.1042/bj3100311.
Abstract
EPR spectroscopy has been successfully used to detect signals due to molybdenum (V) and ferric iron in intact cells of aerobically grown Paracoccus denitrificans. The signals are ascribed to the catalytic molybdenum centre and to the haem iron of the periplasmic nitrate reductase. These signals are absent from a mutant strain deficient in this enzyme. The Mo(V) signal is due to the High-g Split species which has been well characterized in the purified enzyme. This confirms that the High-g Split is the physiologically relevant signal of a number observed in the previous work on the purified enzyme.
摘要
电子顺磁共振光谱已成功用于检测需氧生长的反硝化副球菌完整细胞中钼（V）和三价铁产生的信号。这些信号归因于催化钼中心和周质硝酸还原酶的血红素铁。在缺乏该酶的突变菌株中不存在这些信号。Mo（V）信号归因于高g分裂物种，该物种在纯化的酶中已得到充分表征。这证实了高g分裂是先前对纯化酶的研究中观察到的多个信号中与生理相关的信号。
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