• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Identification of periplasmic nitrate reductase Mo(V) EPR signals in intact cells of Paracoccus denitrificans.在反硝化副球菌完整细胞中鉴定周质硝酸还原酶Mo(V)的电子顺磁共振信号。
Biochem J. 1995 Aug 15;310 ( Pt 1)(Pt 1):311-4. doi: 10.1042/bj3100311.
2
Models for molybdenum coordination during the catalytic cycle of periplasmic nitrate reductase from Paracoccus denitrificans derived from EPR and EXAFS spectroscopy.基于电子顺磁共振（EPR）和扩展X射线吸收精细结构（EXAFS）光谱推导的反硝化副球菌周质硝酸还原酶催化循环中钼配位模型。
Biochemistry. 1999 Jul 13;38(28):9000-12. doi: 10.1021/bi990402n.
3
Mo(V) electron paramagnetic resonance signals from the periplasmic nitrate reductase of Thiosphaera pantotropha.嗜硫代硫杆菌周质硝酸还原酶的钼（Ⅴ）电子顺磁共振信号
Eur J Biochem. 1994 Dec 15;226(3):789-98. doi: 10.1111/j.1432-1033.1994.00789.x.
4
Investigation by electron paramagnetic resonance spectroscopy of the molybdenum centre of respiratory nitrate reductase from Paracoccus denitrificans.利用电子顺磁共振光谱法对反硝化副球菌呼吸硝酸盐还原酶的钼中心进行研究。
Biochem J. 1988 Jun 15;252(3):925-6. doi: 10.1042/bj2520925.
5
Thiocyanate binding to the molybdenum centre of the periplasmic nitrate reductase from Paracoccus pantotrophus.硫氰酸盐与嗜糖假单胞菌周质硝酸还原酶钼中心的结合。
Biochem J. 2000 Dec 15;352 Pt 3(Pt 3):859-64.
6
Mo(V) co-ordination in the periplasmic nitrate reductase from Paracoccus pantotrophus probed by electron nuclear double resonance (ENDOR) spectroscopy.通过电子核双共振（ENDOR）光谱法探测嗜糖假单胞菌周质硝酸还原酶中钼（V）的配位情况。
Biochem J. 2002 May 1;363(Pt 3):817-23. doi: 10.1042/0264-6021:3630817.
7
Purification and characterization of the periplasmic nitrate reductase from Thiosphaera pantotropha.嗜硫代硫酸盐还原菌周质硝酸还原酶的纯化与特性分析
Eur J Biochem. 1994 Feb 15;220(1):117-24. doi: 10.1111/j.1432-1033.1994.tb18605.x.
8
Assignment of haem ligands and detection of electronic absorption bands of molybdenum in the di-haem periplasmic nitrate reductase of Paracoccus pantotrophus.嗜甲基副球菌双血红素周质硝酸还原酶中血红素配体的分配及钼电子吸收带的检测
FEBS Lett. 2001 Jun 29;500(1-2):71-4. doi: 10.1016/s0014-5793(01)02577-7.
9
Purification and initial kinetic and spectroscopic characterization of NO reductase from Paracoccus denitrificans.反硝化副球菌中一氧化氮还原酶的纯化及其初步动力学和光谱表征
Biochim Biophys Acta. 1997 Jan 16;1318(1-2):202-16. doi: 10.1016/s0005-2728(96)00138-7.
10
The bacterial nitrate reductases: EPR studies on nitrate reductase A from Micrococcus denitrificans.细菌硝酸还原酶：对反硝化微球菌硝酸还原酶A的电子顺磁共振研究。
Biochim Biophys Acta. 1972 Feb 28;256(2):600-6. doi: 10.1016/0005-2728(72)90089-8.

引用本文的文献

1
In Vivo EPR Characterization of Semi-Synthetic [FeFe] Hydrogenases.体内半合成 [FeFe]氢化酶的电子顺磁共振特征。
Angew Chem Int Ed Engl. 2018 Mar 1;57(10):2596-2599. doi: 10.1002/anie.201710740. Epub 2018 Feb 6.
2
Mo(V) co-ordination in the periplasmic nitrate reductase from Paracoccus pantotrophus probed by electron nuclear double resonance (ENDOR) spectroscopy.通过电子核双共振（ENDOR）光谱法探测嗜糖假单胞菌周质硝酸还原酶中钼（V）的配位情况。
Biochem J. 2002 May 1;363(Pt 3):817-23. doi: 10.1042/0264-6021:3630817.
3
Molecular genetics of the genus Paracoccus: metabolically versatile bacteria with bioenergetic flexibility.副球菌属的分子遗传学：具有生物能量灵活性的代谢多功能细菌。
Microbiol Mol Biol Rev. 1998 Dec;62(4):1046-78. doi: 10.1128/MMBR.62.4.1046-1078.1998.
4
Cell biology and molecular basis of denitrification.反硝化作用的细胞生物学与分子基础。
Microbiol Mol Biol Rev. 1997 Dec;61(4):533-616. doi: 10.1128/mmbr.61.4.533-616.1997.
5
Structural investigation of the molybdenum site of the periplasmic nitrate reductase from Thiosphaera pantotropha by X-ray absorption spectroscopy.利用X射线吸收光谱法对嗜糖假单胞菌周质硝酸还原酶钼位点的结构研究。
Biochem J. 1996 Jul 15;317 ( Pt 2)(Pt 2):557-63. doi: 10.1042/bj3170557.

本文引用的文献

1
Purification and characterization of the assimilatory nitrate reductase of Azotobacter vinelandii.棕色固氮菌同化型硝酸还原酶的纯化与特性分析
Biochem J. 1993 Jan 15;289 ( Pt 2)(Pt 2):335-42. doi: 10.1042/bj2890335.
2
Characterization of the paramagnetic iron-containing redox centres of Thiosphaera pantotropha periplasmic nitrate reductase.嗜硫代硫酸盐还原菌周质硝酸盐还原酶中含顺磁性铁的氧化还原中心的表征
FEBS Lett. 1994 May 23;345(1):76-80. doi: 10.1016/0014-5793(94)00445-5.
3
Purification and characterization of the periplasmic nitrate reductase from Thiosphaera pantotropha.嗜硫代硫酸盐还原菌周质硝酸还原酶的纯化与特性分析
Eur J Biochem. 1994 Feb 15;220(1):117-24. doi: 10.1111/j.1432-1033.1994.tb18605.x.
4
Multiple states of the molybdenum centre of dimethylsulphoxide reductase from Rhodobacter capsulatus revealed by EPR spectroscopy.通过电子顺磁共振光谱揭示的荚膜红细菌中二甲基亚砜还原酶钼中心的多种状态
Eur J Biochem. 1994 Oct 1;225(1):321-31. doi: 10.1111/j.1432-1033.1994.00321.x.
5
Mo(V) electron paramagnetic resonance signals from the periplasmic nitrate reductase of Thiosphaera pantotropha.嗜硫代硫杆菌周质硝酸还原酶的钼（Ⅴ）电子顺磁共振信号
Eur J Biochem. 1994 Dec 15;226(3):789-98. doi: 10.1111/j.1432-1033.1994.00789.x.
6
The napEDABC gene cluster encoding the periplasmic nitrate reductase system of Thiosphaera pantotropha.编码嗜硫代硫酸盐还原菌周质硝酸还原酶系统的napEDABC基因簇。
Biochem J. 1995 Aug 1;309 ( Pt 3)(Pt 3):983-92. doi: 10.1042/bj3090983.
7
In vitro insertional mutagenesis with a selectable DNA fragment.利用可选择的DNA片段进行体外插入诱变。
Gene. 1984 Sep;29(3):303-13. doi: 10.1016/0378-1119(84)90059-3.
8
The respiratory nitrate reductase from Paracoccus denitrificans. Molecular characterisation and kinetic properties.反硝化副球菌的呼吸硝酸盐还原酶。分子特征及动力学性质。
Eur J Biochem. 1986 Jul 15;158(2):429-36. doi: 10.1111/j.1432-1033.1986.tb09771.x.
9
Respiratory nitrate reductase from Paracoccus denitrificans. Evidence for two b-type haems in the gamma subunit and properties of a water-soluble active enzyme containing alpha and beta subunits.反硝化副球菌的呼吸硝酸盐还原酶。γ亚基中两个b型血红素的证据以及含α和β亚基的水溶性活性酶的性质。
Eur J Biochem. 1988 May 16;174(1):207-12. doi: 10.1111/j.1432-1033.1988.tb14083.x.
10
The inorganic biochemistry of molybdoenzymes.钼酶的无机生物化学
Q Rev Biophys. 1988 Aug;21(3):299-329. doi: 10.1017/s0033583500004479.

在反硝化副球菌完整细胞中鉴定周质硝酸还原酶Mo(V)的电子顺磁共振信号。

Identification of periplasmic nitrate reductase Mo(V) EPR signals in intact cells of Paracoccus denitrificans.

作者信息

Sears H J, Bennett B, Spiro S, Thomson A J, Richardson D J

机构信息

Centre for Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, Norwich, UK.

出版信息

Biochem J. 1995 Aug 15;310 ( Pt 1)(Pt 1):311-4. doi: 10.1042/bj3100311.

DOI:10.1042/bj3100311
PMID:7646461
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1135889/
Abstract

EPR spectroscopy has been successfully used to detect signals due to molybdenum (V) and ferric iron in intact cells of aerobically grown Paracoccus denitrificans. The signals are ascribed to the catalytic molybdenum centre and to the haem iron of the periplasmic nitrate reductase. These signals are absent from a mutant strain deficient in this enzyme. The Mo(V) signal is due to the High-g Split species which has been well characterized in the purified enzyme. This confirms that the High-g Split is the physiologically relevant signal of a number observed in the previous work on the purified enzyme.

摘要

电子顺磁共振光谱已成功用于检测需氧生长的反硝化副球菌完整细胞中钼（V）和三价铁产生的信号。这些信号归因于催化钼中心和周质硝酸还原酶的血红素铁。在缺乏该酶的突变菌株中不存在这些信号。Mo（V）信号归因于高g分裂物种，该物种在纯化的酶中已得到充分表征。这证实了高g分裂是先前对纯化酶的研究中观察到的多个信号中与生理相关的信号。