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[薄层扫描法测定复方中药制剂中熊去氧胆酸和鹅去氧胆酸的含量]

[Determination of ursodeoxycholic acid and chenodeoxycholic acid in Chinese medicinal compound preparations by TLC-densitometry].

作者信息

Zhang Z, Liao G, Song Y, Yin H

机构信息

School of Pharmacy, West China University of Medical Sciences, Chengdu.

出版信息

Zhongguo Zhong Yao Za Zhi. 1995 Mar;20(3):162-4, 192.

PMID:7646779
Abstract

The contents of ursodeoxycholic acid (UDCA) and chenodeoxycholic acid (CDCA) in Chinese medicinal compound preparations have been determined by TLC-densitometry. The determination wavelength was 375nm and reference wavelength was 700nm. For UDCA the linear range was 3.0-15.0 micrograms and recovery was 97.2% (RSD = 1.2%), and for CDCA the linear range was 4.0-20.0 micrograms and recovery was 99.3% (RSD = 2.1%).

摘要

采用薄层扫描法测定了中药复方制剂中熊去氧胆酸(UDCA)和鹅去氧胆酸(CDCA)的含量。测定波长为375nm,参比波长为700nm。熊去氧胆酸的线性范围为3.0 - 15.0微克,回收率为97.2%(相对标准偏差RSD = 1.2%);鹅去氧胆酸的线性范围为4.0 - 20.0微克,回收率为99.3%(相对标准偏差RSD = 2.1%)。

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