使用全内反射荧光法（TIRF）测定吸附荧光团的取向分布。I. 理论
Determination of the orientation distribution of adsorbed fluorophores using TIRF. I. Theory.
作者信息
Bos M A, Kleijn J M
机构信息
Department of Physical and Colloid Chemistry, Agricultural University, Wageningen, The Netherlands.
出版信息
Biophys J. 1995 Jun;68(6):2566-72. doi: 10.1016/S0006-3495(95)80439-X.
Abstract
The spectroscopic technique total internal reflection fluorescence can be used for determination of the orientation of adsorbed fluorescent molecules. The underlying theory is presented in general terms and elaborated in detail for the case that the fluorescent group is a porphyrin ring. It is shown that order parameters of the orientation distribution can be obtained if both the fluorescence intensity and its polarization are measured as functions of the polarization of the incident laser beam. From these order parameters an approximation of the orientation distribution can be derived by the maximum-entropy method.
摘要
全内反射荧光光谱技术可用于测定吸附荧光分子的取向。文中概述了其基本理论，并针对荧光基团为卟啉环的情况进行了详细阐述。结果表明，如果同时测量荧光强度及其偏振度随入射激光束偏振度的变化，就可以得到取向分布的序参量。通过最大熵方法，可从这些序参量推导出取向分布的近似值。
相似文献
1
Determination of the orientation distribution of adsorbed fluorophores using TIRF. I. Theory.使用全内反射荧光法（TIRF）测定吸附荧光团的取向分布。I. 理论
Biophys J. 1995 Jun;68(6):2566-72. doi: 10.1016/S0006-3495(95)80439-X.
2
Determination of the orientation distribution of adsorbed fluorophores using TIRF. II. Measurements on porphyrin and cytochrome c.使用全内反射荧光法测定吸附荧光团的取向分布。II. 卟啉和细胞色素c的测量
Biophys J. 1995 Jun;68(6):2573-9. doi: 10.1016/S0006-3495(95)80440-6.
3
Orientation of adsorbed cytochrome c as a function of the electrical potential of the interface studied by total internal reflection fluorescence.通过全内反射荧光研究吸附的细胞色素c的取向与界面电势的关系。
Biophys J. 1990 May;57(5):965-75. doi: 10.1016/S0006-3495(90)82616-3.
4
Adsorption of a protein-porphyrin complex at a liquid-liquid interface studied by total internal reflection synchronous fluorescence spectroscopy.通过全内反射同步荧光光谱法研究蛋白质-卟啉复合物在液-液界面的吸附
Anal Chim Acta. 2008 Apr 28;614(1):71-6. doi: 10.1016/j.aca.2008.03.017. Epub 2008 Mar 14.
5
Interaction between certain porphyrins and CdS colloids: a steady state and time resolved fluorescence quenching study.某些卟啉与硫化镉胶体之间的相互作用：稳态及时间分辨荧光猝灭研究
Spectrochim Acta A Mol Biomol Spectrosc. 2008 Dec 15;71(4):1507-11. doi: 10.1016/j.saa.2008.05.011. Epub 2008 May 18.
6
Total internal reflection fluorescence. Measurement of spatial and orientational distributions of fluorophores near planar dielectric interfaces.
Biophys Chem. 1986 Nov;25(1):91-7. doi: 10.1016/0301-4622(86)85069-4.
7
Measuring surface dynamics of biomolecules by total internal reflection fluorescence with photobleaching recovery or correlation spectroscopy.通过光漂白恢复或相关光谱的全内反射荧光测量生物分子的表面动力学。
Biophys J. 1981 Mar;33(3):435-54. doi: 10.1016/S0006-3495(81)84905-3.
8
Theory of fluorescence polarization of oriented pigment molecules in spherical arrays.
Biophys Struct Mech. 1976 Dec 22;2(3):243-50. doi: 10.1007/BF00535370.
9
Fluorescence depolarization measurements on oriented membranes.对取向膜进行荧光去极化测量。
Biophys J. 1988 Jun;53(6):989-1005. doi: 10.1016/S0006-3495(88)83179-5.
10
Fluorescence recovery spectroscopy as a probe of slow rotational motions.荧光恢复光谱法作为慢旋转运动的一种探测手段。
Biophys J. 1984 Dec;46(6):795-803. doi: 10.1016/S0006-3495(84)84078-3.
引用本文的文献
1
A general orientation distribution function for clay-rich media.一种适用于富黏土介质的通用取向分布函数。
Nat Commun. 2019 Nov 29;10(1):5456. doi: 10.1038/s41467-019-13401-0.
2
3D fluorescence anisotropy imaging using selective plane illumination microscopy.使用选择性平面照明显微镜的三维荧光各向异性成像
Opt Express. 2015 Aug 24;23(17):22308-17. doi: 10.1364/OE.23.022308.
3
Fluorescence polarization/anisotropy in diagnostics and imaging.诊断与成像中的荧光偏振/各向异性
Chem Rev. 2010 May 12;110(5):2685-708. doi: 10.1021/cr900267p.
4
Effects of surface wettability and contact time on protein adhesion to biomaterial surfaces.表面润湿性和接触时间对蛋白质黏附于生物材料表面的影响。
Biomaterials. 2007 Aug;28(22):3273-83. doi: 10.1016/j.biomaterials.2007.03.032. Epub 2007 Apr 12.
5
Order in phospholipid Langmuir-Blodgett layers and the effect of the electrical potential of the substrate.磷脂朗缪尔-布洛杰特膜中的有序性及基底电势的影响
Biophys J. 1999 Jan;76(1 Pt 1):323-32. doi: 10.1016/S0006-3495(99)77199-7.
6
Order in phospholipid Langmuir-Blodgett monolayers determined by total internal reflection fluorescence.通过全内反射荧光测定磷脂朗缪尔-布洛杰特单分子层中的有序性。
Biophys J. 1997 Jun;72(6):2651-9. doi: 10.1016/S0006-3495(97)78908-2.
本文引用的文献
1
Surface order and stability of langmuir-blodgett films.朗缪尔-布洛杰特膜的表面有序性与稳定性
Science. 1992 Jul 24;257(5069):508-11. doi: 10.1126/science.257.5069.508.
2
Order in supported phospholipid monolayers detected by the dichroism of fluorescence excited with polarized evanescent illumination.通过偏振倏逝光激发荧光的二色性检测支持的磷脂单层中的有序性。
Biophys J. 1984 Dec;46(6):739-47. doi: 10.1016/S0006-3495(84)84072-2.
3
Effect of planar dielectric interfaces on fluorescence emission and detection. Evanescent excitation with high-aperture collection.平面介电界面对荧光发射和检测的影响。采用高孔径收集的倏逝激发。
Biophys J. 1984 Dec;46(6):729-37. doi: 10.1016/S0006-3495(84)84071-0.
4
Order and dynamics in the lamellar L alpha and in the hexagonal HII phase. Dioleoylphosphatidylethanolamine studied with angle-resolved fluorescence depolarization.层状Lα相和六方HII相中的有序性与动力学。用角分辨荧光去极化研究二油酰磷脂酰乙醇胺。
Biophys J. 1989 May;55(5):937-47. doi: 10.1016/S0006-3495(89)82892-9.
5
Determination of protein orientation on surfaces with X-ray photoelectron spectroscopy.
J Protein Chem. 1990 Feb;9(1):105-8. doi: 10.1007/BF01024991.
6
Orientation of adsorbed cytochrome c as a function of the electrical potential of the interface studied by total internal reflection fluorescence.通过全内反射荧光研究吸附的细胞色素c的取向与界面电势的关系。
Biophys J. 1990 May;57(5):965-75. doi: 10.1016/S0006-3495(90)82616-3.
7
Carbocyanine dye orientation in red cell membrane studied by microscopic fluorescence polarization.通过显微荧光偏振研究红细胞膜中花青染料的取向。
Biophys J. 1979 Jun;26(3):557-73. doi: 10.1016/S0006-3495(79)85271-6.
8
Metallocytochromes c: characterization of electronic absorption and emission spectra of Sn4+ and Zn2+ cytochromes c.金属细胞色素c：Sn4+和Zn2+细胞色素c的电子吸收光谱和发射光谱特征
Eur J Biochem. 1976 May 1;64(2):381-7. doi: 10.1111/j.1432-1033.1976.tb10312.x.
9
Cytochrome c interaction with membranes. Absorption and emission spectra and binding characteristics of iron-free cytochrome c.细胞色素c与膜的相互作用。无铁细胞色素c的吸收光谱、发射光谱及结合特性。
Eur J Biochem. 1975 Dec 1;60(1):199-207. doi: 10.1111/j.1432-1033.1975.tb20992.x.
Zotero 插件