B 细胞慢性淋巴细胞白血病患者淋巴细胞中的嘌呤核苷酸代谢

Purine nucleotide metabolism in lymphocytes of B-cell chronic lymphocytic leukemia patients.

作者信息

Tabucchi A, Carlucci F, Leoncini R, Vannoni D, Consolmagno E, Marinello E, Pizzichini M, Dispensa E, Pagani R

机构信息

Istituto di Biochimica e di Enzimologia, Università di Siena, Italy.

出版信息

Biomed Pharmacother. 1995;49(3):141-4. doi: 10.1016/0753-3322(96)82607-2.

DOI:10.1016/0753-3322(96)82607-2

PMID:7647285

Abstract

Purine nucleotides were studied in human peripheral blood lymphocytes from normal subjects and patients with chronic B-cell lymphocytic leukemia (B-CLL). Nucleotide content was determined by high performance liquid chromatography (HPLC). The overall rate of purine nucleotide synthesis was measured following the incorporation of 14C-formate into the nucleotides of a lymphocytic suspension. Results indicate a substantially reduced rate of purine nucleotide metabolism.

摘要

对正常受试者以及慢性B淋巴细胞白血病（B-CLL）患者的人外周血淋巴细胞中的嘌呤核苷酸进行了研究。通过高效液相色谱法（HPLC）测定核苷酸含量。在将14C-甲酸掺入淋巴细胞悬液的核苷酸中之后，测量嘌呤核苷酸合成的总体速率。结果表明嘌呤核苷酸代谢速率大幅降低。