感染HIV-1病毒的淋巴细胞中游离嘌呤核苷酸的行为。

The behavior of free purine nucleotides in lymphocytes infected with HIV-1 virus.

作者信息

Tabucchi A, Carlucci F, Re M C, Furlini G, Consolmagno E, Leoncini R, Pizzichini M, Marinello E, Rubino M, Pagani R

机构信息

Istituto di Biochimica e di Enzimologia, Università di Siena, Italy.

出版信息

Biochim Biophys Acta. 1993 Oct 20;1182(3):317-22. doi: 10.1016/0925-4439(93)90075-c.

DOI:10.1016/0925-4439(93)90075-c

PMID:8399367

Abstract

The purine nucleotide content was examined in various cells before and after HIV-1 virus infection: healthy peripheral blood lymphocytes (PBL) and cultured PBL after infection; the PBL of asymptomatic, ARC and AIDS patients. In all cases, changes in purine nucleotide concentrations were observed. The pattern of purine nucleosides and nucleobases was also evaluated by HPLC in PBL of controls and patients. The analysis was integrated by following the incorporation of a labelled precursor ([14C]formate) into purine nucleotides, which was investigated as an indication of the rate of purine metabolism in these cells. Many interesting variations in the catabolic and of anabolic pathways were observed, demonstrating that the viral penetration affects purine nucleotide metabolism. These results suggest interesting perspectives in AIDS research.

摘要

在HIV-1病毒感染前后，对各种细胞中的嘌呤核苷酸含量进行了检测：健康外周血淋巴细胞（PBL）以及感染后的培养PBL；无症状、患艾滋病相关综合征（ARC）和艾滋病患者的PBL。在所有情况下，均观察到嘌呤核苷酸浓度的变化。还通过高效液相色谱法（HPLC）对对照组和患者PBL中的嘌呤核苷和碱基模式进行了评估。通过追踪标记前体（[14C]甲酸）掺入嘌呤核苷酸的情况来整合分析，将其作为这些细胞中嘌呤代谢速率的指标进行研究。观察到分解代谢和合成代谢途径存在许多有趣的变化，表明病毒侵入会影响嘌呤核苷酸代谢。这些结果为艾滋病研究提供了有趣的视角。