Ye P, Chen B, Wang S
Chinese PLA General Hospital, Beijing.
Chin Med Sci J. 1995 Jun;10(2):63-9.
The polymorphisms of variable number of tandem repeats (VNTR) 3' to the apolipoprotein B (apo B) gene were investigated using polymerase chain reaction (PCR) in a sample of 103 patients with documented coronary heart disease (CHD) and 100 healthy individuals selected from Chinese Han nationality. Twelve segregating alleles (3' beta 29-51) were observed in the pooled total of 203 subjects. The most common allele was 3' beta 37, followed by 3' beta 39 with frequencies of 0.362 and 0.296, respectively. This model of allele distribution was coincident with the results form different ethnic groups, but the relative frequencies of alleles were different. In comparison with the allele frequencies between the patients and controls, alleles bigger than 3' beta 39 (3' VNTR-B) were significantly more common among the patients than among the controls (P < 0.001). Moreover, in the CHD group patients with plasma levels of TC > or = 3.88 mmol/L, LDL-C > or = 2.59 mmol/L and HDL-C < 1.16 mmol/L had significantly higher frequencies of 3' VNTR-B allele (P < 0.01). Therefore, it is suggested that 3' VNTR-B allele might be involved in the development of coronary atherosclerosis, presumably through their influences on lipid metabolism.
运用聚合酶链反应（PCR）技术，对103例有冠心病（CHD）病史的患者及100名来自中国汉族的健康个体进行研究，以检测载脂蛋白B（apo B）基因3'端可变数目串联重复序列（VNTR）的多态性。在总共203名受试者中观察到12个分离等位基因（3'β29 - 51）。最常见的等位基因是3'β37，其次是3'β39，频率分别为0.362和0.296。这种等位基因分布模式与不同种族群体的结果一致，但等位基因的相对频率有所不同。与患者和对照组的等位基因频率相比，大于3'β39的等位基因（3'VNTR - B）在患者中显著比对照组更常见（P < 0.001）。此外，在CHD组中，血浆总胆固醇（TC）≥3.88 mmol/L、低密度脂蛋白胆固醇（LDL - C）≥2.59 mmol/L且高密度脂蛋白胆固醇（HDL - C）< 1.16 mmol/L的患者中，3'VNTR - B等位基因的频率显著更高（P < 0.01）。因此，提示3'VNTR - B等位基因可能参与冠状动脉粥样硬化的发生发展，可能是通过其对脂质代谢的影响。
