Komaki K, Kamamura Y, Ohmine Y, Sasa M, Tanaka K, Inoue H, Uyama T, Morimoto T, Monden Y
2nd Department of Surgery, School of Medicine, University of Tokushima, Japan.
Breast Cancer Res Treat. 1995 Aug;35(2):157-62. doi: 10.1007/BF00668205.
Thymidylate synthetase (TS) is a key enzyme as a methyl donor in the methylation reaction from dUMP to dTMP. TS activity was assessed in various tissue of mammary disorders. The descending order of TS activity was as follows: cancer-positive nodes, primary cancers, cancer-negative nodes, benign lesions, and normal parenchyma. Significant differences in TS activity were found between the positive nodes and each of the other tissues (p < 0.01). In node-positive cases, a significant correlation in TS activity was found between the primary cancers and positive nodes (r = 0.616, p = 0.033). There was no correlation between the nodal status and the TS activity in primary cancers. In 11 of 12 cases, the TS activity of positive nodes was higher than the 'calculated' TS activity of the primary cancer, which was defined as the TS activity per unit weight of cancer cells. A significant correlation was found between the calculated TS activity and the mitotic frequency in primary cancers (r = 0.697, p = 0.0001). On the other hand, a significant correlation could not be found between the TS activity and the mitotic frequency in positive nodes (r = 0.364, p = 0.244).
胸苷酸合成酶（TS）是在从脱氧尿苷酸（dUMP）到脱氧胸苷酸（dTMP）的甲基化反应中作为甲基供体的关键酶。在各种乳腺疾病组织中评估了TS活性。TS活性由高到低依次为：癌阳性淋巴结、原发性癌、癌阴性淋巴结、良性病变和正常实质。在阳性淋巴结与其他每种组织之间发现TS活性存在显著差异（p < 0.01）。在淋巴结阳性病例中，原发性癌与阳性淋巴结之间的TS活性存在显著相关性（r = 0.616，p = 0.033）。原发性癌的淋巴结状态与TS活性之间无相关性。在12例中的11例中，阳性淋巴结的TS活性高于原发性癌的“计算”TS活性，“计算”TS活性定义为每单位重量癌细胞的TS活性。在原发性癌中，计算得到的TS活性与有丝分裂频率之间存在显著相关性（r = 0.697，p = 0.0001）。另一方面，在阳性淋巴结中，TS活性与有丝分裂频率之间未发现显著相关性（r = 0.364，p = 0.244）。
