Lebbar S, Haeffner-Cavaillon N, Karibian D, Le Beyec Y, Caroff M
Equipe 'Endotoxines', URA 1116 du Centre National de la Recherche Scientifique, Biochimie, Université de Paris-Sud, Orsay, France.
Rapid Commun Mass Spectrom. 1995;9(8):693-6. doi: 10.1002/rcm.1290090812.
Lipids A are the hydrophobic domains of bacterial endotoxic lipopolysaccharides. Since they are responsible for most of the biological activities (both pathogenic and beneficial) of endotoxins, the characterization of their structure is crucial to the understanding of their mode of action. However, the inadequacy of existing methods for preparing certain lipids A has prompted us to devise a new, mild procedure which gives intact products. Use was made of the special features of 252Cf-plasma desorption mass spectrometry for forming molecular ions from these species and giving qualitative and quantitative information from the primary mass spectrum.
类脂A是细菌内毒素脂多糖的疏水结构域。由于它们是内毒素大多数生物活性（包括致病和有益活性）的原因，对其结构的表征对于理解其作用方式至关重要。然而，现有制备某些类脂A方法的不足促使我们设计一种新的温和方法，该方法能得到完整的产物。利用252Cf等离子体解吸质谱的特殊功能，从这些物质中形成分子离子，并从一级质谱中提供定性和定量信息。
