一种双生病毒可在终末分化的植物细胞中诱导宿主DNA合成蛋白的表达。
A geminivirus induces expression of a host DNA synthesis protein in terminally differentiated plant cells.
作者信息
Nagar S, Pedersen T J, Carrick K M, Hanley-Bowdoin L, Robertson D
机构信息
Department of Botany, North Carolina State University, Raleigh 27695-7612, USA.
出版信息
Plant Cell. 1995 Jun;7(6):705-19. doi: 10.1105/tpc.7.6.705.
Abstract
Geminiviruses are plant DNA viruses that replicate through DNA intermediates in plant nuclei. The viral components required for replication are known, but no host factors have yet been identified. We used immunolocalization to show that the replication proteins of the geminivirus tomato golden mosaic virus (TGMV) are located in nuclei of terminally differentiated cells that have left the cell cycle. In addition, TGMV infection resulted in a significant accumulation of the host DNA synthesis protein proliferating cell nuclear antigen (PCNA). PCNA, an accessory factor for DNA polymerase delta, was not present at detectable levels in healthy differentiated cells. The TGMV replication protein AL1 was sufficient to induce accumulation of PCNA in terminally differentiated cells of transgenic plants. Analysis of the mechanism(s) whereby AL1 induces the accumulation of host replication machinery in quiescent plant cells will provide a unique opportunity to study plant DNA synthesis.
摘要
双生病毒是一类植物DNA病毒，它们通过DNA中间体在植物细胞核中进行复制。已知复制所需的病毒成分，但尚未鉴定出宿主因子。我们利用免疫定位技术表明，双生病毒番茄金色花叶病毒（TGMV）的复制蛋白位于已退出细胞周期的终末分化细胞的细胞核中。此外，TGMV感染导致宿主DNA合成蛋白增殖细胞核抗原（PCNA）大量积累。PCNA是DNA聚合酶δ的辅助因子，在健康的分化细胞中检测不到其存在。TGMV复制蛋白AL1足以在转基因植物的终末分化细胞中诱导PCNA积累。分析AL1在静止植物细胞中诱导宿主复制机制积累的机制，将为研究植物DNA合成提供独特的机会。
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A geminivirus induces expression of a host DNA synthesis protein in terminally differentiated plant cells.一种双生病毒可在终末分化的植物细胞中诱导宿主DNA合成蛋白的表达。
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本文引用的文献
1
Complete nucleotide sequence of the infectious cloned DNA components of tomato golden mosaic virus: potential coding regions and regulatory sequences.番茄金黄镶嵌病毒感染性克隆 DNA 组分的全核苷酸序列：潜在的编码区和调控序列。
EMBO J. 1984 Sep;3(9):2197-205. doi: 10.1002/j.1460-2075.1984.tb02114.x.
2
Plasmodesmata: the intercellular organelles of green plants.胞间连丝：绿色植物的细胞间细胞器。
Trends Cell Biol. 1993 Sep;3(9):308-15. doi: 10.1016/0962-8924(93)90013-q.
3
Localization of the Functional p34cdc2 Homolog of Maize in Root Tip and Stomatal Complex Cells: Association with Predicted Division Sites.玉米功能性p34cdc2同源物在根尖和气孔复合体细胞中的定位：与预测分裂位点的关联
Plant Cell. 1993 Sep;5(9):1101-1111. doi: 10.1105/tpc.5.9.1101.
4
Replication of maize streak virus mutants in maize protoplasts: evidence for a movement protein.玉米条纹病毒突变体在玉米原生质体中的复制：运动蛋白的证据
Virology. 1993 Jan;192(1):85-93. doi: 10.1006/viro.1993.1010.
5
Cell division in plants.植物中的细胞分裂。
Curr Opin Cell Biol. 1993 Apr;5(2):226-31. doi: 10.1016/0955-0674(93)90107-2.
6
Reversal of terminal differentiation and control of DNA replication: cyclin A and Cdk2 specifically localize at subnuclear sites of DNA replication.终末分化的逆转与DNA复制的控制：细胞周期蛋白A和细胞周期蛋白依赖性激酶2特异性定位于DNA复制的亚核位点。
Cell. 1993 Sep 24;74(6):979-92. doi: 10.1016/0092-8674(93)90721-2.
7
Digitaria streak geminivirus replicative forms are abundant in S-phase nuclei of infected cells.马唐条纹双生病毒复制形式在受感染细胞的S期细胞核中大量存在。
Virology. 1993 Jul;195(1):257-9. doi: 10.1006/viro.1993.1369.
8
Patterns of cell division revealed by transcriptional regulation of genes during the cell cycle in plants.植物细胞周期中基因转录调控所揭示的细胞分裂模式。
EMBO J. 1994 Feb 1;13(3):616-24. doi: 10.1002/j.1460-2075.1994.tb06299.x.
9
cdc2a expression in Arabidopsis is linked with competence for cell division.拟南芥中cdc2a的表达与细胞分裂能力相关。
Plant Cell. 1993 Dec;5(12):1711-23. doi: 10.1105/tpc.5.12.1711.
10
Geminivirus replication origins have a modular organization.双生病毒复制起点具有模块化结构。
Plant Cell. 1994 Mar;6(3):405-16. doi: 10.1105/tpc.6.3.405.
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