Krémarik P, Freund-Mercier M J, Stoeckel M E
Laboratoire de Physiologie Générale, URA CNRS 1446, Strasbourg, France.
J Neuroendocrinol. 1995 Apr;7(4):281-9. doi: 10.1111/j.1365-2826.1995.tb00759.x.
The localization at the cellular level and the regulation by progesterone of the estrogen-sensitive oxytocin binding sites was studied in the rat telencephalon and the hypothalamus by using quantitative film-autoradiography and histoautoradiography. Male rats (castrated or not) and ovariectomized females (estradiol supplemented or not) were used to characterize these sites and to precise their localization. They were detected in the striatal cell bridges, the olfactory tubercle, the principal nucleus of the bed nucleus of the stria terminalis and the medial nucleus of the amygdala of the telencephalon and in the medial preoptic, the ventromedial and the ventral premammillary nuclei of the hypothalamus. Estrogen administration in addition induced expression of oxytocin binding sites in the major island of Calleja, the anterior hypothalamic area and the terete nucleus. The density of the estrogen-sensitive oxytocin binding sites varied during the estrous cycle, but differently in the telencephalon and the hypothalamus. In the telencephalon it peaked at proestrus 9 h and was already decreased at proestrus 21 h, whereas in the hypothalamus it was similarly high at proestrus 9 h and proestrus 21 h, suggesting the intervention of progesterone in the regulation of the hypothalamic estrogen-sensitive oxytocin binding sites.
利用定量放射自显影术和组织放射自显影术，在大鼠端脑和下丘脑研究了雌激素敏感的催产素结合位点在细胞水平的定位及其受孕酮的调节。使用雄性大鼠（阉割与否）和去卵巢雌性大鼠（补充雌二醇与否）来表征这些位点并明确其定位。在端脑的纹状体细胞桥、嗅结节、终纹床核主核和杏仁核内侧核以及下丘脑的内侧视前核、腹内侧核和腹侧乳头前核中检测到了这些位点。此外，给予雌激素还诱导了在主要的卡勒哈岛、下丘脑前部区域和圆柱核中催产素结合位点的表达。雌激素敏感的催产素结合位点的密度在发情周期中有所变化，但在端脑和下丘脑中变化不同。在端脑中，它在发情前期9小时达到峰值，而在发情前期21小时已经下降，而在下丘脑中，它在发情前期9小时和发情前期21小时同样高，这表明孕酮参与了下丘脑雌激素敏感的催产素结合位点的调节。
