Dabike M, Munizaga A, Koenig C S
Departamento de Biología Celular y Molecular, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago.
Biol Res. 1994;27(1):29-38.
The association of myosin and a filamin-like protein to the F-actin cytoskeleton of parietal cells was studied in the rat gastric mucosa. Myosin and the filamin-like protein were localized by indirect immunofluorescence microscopy while the distribution of actin was established by using FITC-phalloidin. These cytoskeletal proteins, concentrated in the parietal cells, changed their distribution in correlation with the hydrochloric acid (HCl) secretory state of the cells and the appearance of a developed intracellular canaliculus. Thus, in resting parietal cells, actin showed a patchy distribution, delimiting the poorly developed secretory canaliculi, while myosin and the filamin-like protein distributed diffusely over the cytoplasm. In secreting cells, F-actin was concentrated in the cytoplasmic projections filling the canalicular lumen, while myosin and the filamin-like protein were excluded from this region, concentrating in the adjoining cytoplasm. The present results show that myosin and the filamin-like protein change their association with the secretory membranes in relation to the development of the secretory canaliculus of parietal cells. In resting cells, both proteins associate with the endocellular secretory membranes. In secreting cells, the microvillar projections of the canalicular surface formed by these membranes bind F-actin, but exclude myosin and the filamin-like protein.
在大鼠胃黏膜中研究了肌球蛋白和一种细丝蛋白样蛋白与壁细胞F-肌动蛋白细胞骨架的关联。通过间接免疫荧光显微镜对肌球蛋白和细丝蛋白样蛋白进行定位，同时使用异硫氰酸荧光素（FITC）标记的鬼笔环肽确定肌动蛋白的分布。这些细胞骨架蛋白集中在壁细胞中，其分布随着细胞的盐酸（HCl）分泌状态和发达的细胞内小管的出现而改变。因此，在静息壁细胞中，肌动蛋白呈斑片状分布，界定发育不良的分泌小管，而肌球蛋白和细丝蛋白样蛋白则在细胞质中呈弥漫性分布。在分泌细胞中，F-肌动蛋白集中在充满小管腔的细胞质突起中，而肌球蛋白和细丝蛋白样蛋白则被排除在该区域，集中在相邻的细胞质中。目前的结果表明，肌球蛋白和细丝蛋白样蛋白与分泌膜的关联随着壁细胞分泌小管的发育而改变。在静息细胞中，这两种蛋白都与细胞内分泌膜相关联。在分泌细胞中，由这些膜形成的小管表面的微绒毛突起结合F-肌动蛋白，但排除肌球蛋白和细丝蛋白样蛋白。
