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猪痢疾密螺旋体（猪痢疾蛇形螺旋体）在铁限制条件下的生长

Growth of Serpulina (Treponema) hyodysenteriae under iron-restricted conditions.

作者信息

Li Z, Foiry B, Jacques M

机构信息

Département de Pathologie et Microbiologie, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec.

出版信息

Can J Vet Res. 1995 Apr;59(2):149-53.

PMID:7648528
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1263753/
Abstract

Reference strains of Serpulina hyodysenteriae expressed at least three iron-regulated proteins with apparent molecular masses of > 200, 134, and 109 kDa when grown under iron-restricted conditions. Cells of S. hyodysenteriae grown under these conditions also showed increased outer membrane bleb formation when examined by electron microscopy after negative staining. S. hyodysenteriae did not use the 2 most common types of siderophore, namely catechol and hydroxamate. Western blotting with serum from a pig experimentally infected with S. hyodysenteriae B204 indicated that the 109-kDa major iron-regulated protein was expressed in vivo and was conserved among all strains tested.

摘要

在铁限制条件下生长时，猪痢疾蛇形螺旋体参考菌株表达至少三种铁调节蛋白，其表观分子量分别大于200 kDa、134 kDa和109 kDa。在这些条件下生长的猪痢疾蛇形螺旋体细胞经负染后通过电子显微镜检查，也显示出外膜泡形成增加。猪痢疾蛇形螺旋体不利用两种最常见的铁载体类型，即儿茶酚和异羟肟酸。用实验感染猪痢疾蛇形螺旋体B204的猪血清进行蛋白质印迹分析表明，109 kDa的主要铁调节蛋白在体内表达，并且在所测试的所有菌株中都保守。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2692/1263753/f45439264110/cjvetres00026-0074-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2692/1263753/0bc547dc49f2/cjvetres00026-0072-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2692/1263753/85c8cd030ace/cjvetres00026-0073-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2692/1263753/591e715b15cf/cjvetres00026-0073-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2692/1263753/f45439264110/cjvetres00026-0074-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2692/1263753/0bc547dc49f2/cjvetres00026-0072-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2692/1263753/85c8cd030ace/cjvetres00026-0073-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2692/1263753/591e715b15cf/cjvetres00026-0073-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2692/1263753/f45439264110/cjvetres00026-0074-a.jpg

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本文引用的文献

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J Bacteriol. 1993 May;175(10):2980-7. doi: 10.1128/jb.175.10.2980-2987.1993.
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