Chen K, Lin Y, Chen G
Fujian Provincial Health and Anti-epidemic Station, Fuzhou.
Zhonghua Yu Fang Yi Xue Za Zhi. 1995 May;29(3):138-40.
Species of Vibrio fluvialis are identified by their biochemical characteristics so far, with a lot of items to be determined, overelaborate procedure and expensiveness. Inaccordance with the fact that bacteriophage is a specific parasite inhabited in bacteria and has been used in identifying other bacteria with high specificity, some of Vibrio fluvialis bacteriophage isolated from natural environment were selected to make diagnostic preparation for Vibrio fluvialis identification. Diagnostic positivity of Vibrio fluvialis averaged 84.27%, and 87.84% for those of human source. Cross-lysis rates both for the same Vibrio genus and that of different families in the same genus were less then 3%, and no cross-lysis was found for the other enteric bacteria in different genera. There was no significant difference between diagnostic bacteriophage and biochemical tests for Vibrio of unknown species. This method was highly specific, sensitive, rapid, simple and inexpensive, and could be used in diagnosis of Vibrio fluvialis.
目前，河流弧菌的鉴定主要依据其生化特性，然而需要测定的项目众多，操作流程繁琐且成本高昂。鉴于噬菌体是寄生于细菌的特异性寄生虫，已被用于高特异性地鉴定其他细菌，因此从自然环境中分离出一些河流弧菌噬菌体，用于制备河流弧菌鉴定的诊断制剂。河流弧菌的诊断阳性率平均为84.27%，来源于人类的菌株诊断阳性率为87.84%。同一弧菌属内以及同属不同菌科之间的交叉裂解率均低于3%，不同属的其他肠道细菌未发现交叉裂解现象。对于未知弧菌的诊断，诊断噬菌体与生化试验之间无显著差异。该方法具有高度特异性、敏感性、快速、简便且成本低廉，可用于河流弧菌的诊断。
