硫酸角质素的13C核磁共振光谱。源自牛关节软骨硫酸角质素经角质酶II消化得到的四种聚（N-乙酰乳糖胺）重复序列四糖的归属。
13C-NMR spectroscopy of keratan sulphates. Assignments for four poly(N-acetyllactosamine)-repeat-sequence tetrasaccharides derived from bovine articular cartilage keratan sulphate by keratanase II digestion.
作者信息
Huckerby T N, Brown G M, Nieduszynski I A
机构信息
Polymer Centre, School of Physics and Chemistry, Lancaster University, England.
出版信息
Eur J Biochem. 1995 Aug 1;231(3):779-83. doi: 10.1111/j.1432-1033.1995.0779d.x.
Skeletal keratan sulphate has been fragmented using the enzyme keratanase II, and the complete 13C chemical shift data are reported for the four tetrasaccharides which derived from the repeat region. They have the structures: Gal beta(1-4)GlcNAc(6S)beta(1-3)Gal beta(1-4)GlcNAc(6S)-ol, Gal(6S)beta(1-4)GlcNAc(6S)beta(1-3)Gal(1-4)GlcNAc(6S)-ol, Gal beta(1-4)GlcNAc(6S)beta(1-3)Gal(6S)beta(1-4)GlcNAc(6S)-ol, and Gal(6S)beta(1-4)GlcNAc(6S)(1-3)Gal(6S)beta(1-4)GlcNAc(6S)-ol, where GlcNAc(6S)-ol represents N-acetyl-glucosaminitol-6-O-sulphate. The value of these data for microstructural analysis of undegraded keratan sulphate samples using 13C-NMR spectroscopy is discussed with respect to galactose sulphation levels and the block sulphation structure.
硫酸角质素已用角质酶II进行了片段化处理，并报告了源自重复区域的四种四糖的完整13C化学位移数据。它们具有以下结构：Gal β(1-4)GlcNAc(6S)β(1-3)Gal β(1-4)GlcNAc(6S)-ol、Gal(6S)β(1-4)GlcNAc(6S)β(1-3)Gal(1-4)GlcNAc(6S)-ol、Gal β(1-4)GlcNAc(6S)β(1-3)Gal(6S)β(1-4)GlcNAc(6S)-ol和Gal(6S)β(1-4)GlcNAc(6S)(1-3)Gal(6S)β(1-4)GlcNAc(6S)-ol，其中GlcNAc(6S)-ol代表N-乙酰葡糖胺醇-6-O-硫酸盐。关于半乳糖硫酸化水平和嵌段硫酸化结构，讨论了这些数据对于使用13C-NMR光谱对未降解硫酸角质素样品进行微观结构分析的价值。
Zotero 插件