Tachikawa H, Takeuchi Y, Funahashi W, Miura T, Gao X D, Fujimoto D, Mizunaga T, Onodera K
Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan.
FEBS Lett. 1995 Aug 7;369(2-3):212-6. doi: 10.1016/0014-5793(95)00750-4.
MPD1, a yeast gene the overexpression of which suppresses the inviability caused by the loss of protein disulfide isomerase (PDI) was isolated and characterized. The MPD1 gene product retained a single disulfide isomerase active site sequence (APWCGHCK), an N-terminal putative signal sequence, and a C-terminal endoplasmic reticulum (ER) retention signal, and was a novel member of the PDI family. The gene product, identified in yeast extract, contained core size carbohydrates. MPD1 was not essential for growth, but overexpression of the gene suppressed the maturation defect of carboxypeptidase Y caused by PDI1 deletion, indicative of the related function to PDI in the yeast ER.
分离并鉴定了MPD1，这是一种酵母基因，其过表达可抑制因蛋白质二硫键异构酶（PDI）缺失而导致的致死性。MPD1基因产物保留了一个单一的二硫键异构酶活性位点序列（APWCGHCK）、一个N端推定信号序列和一个C端内质网（ER）滞留信号，是PDI家族的一个新成员。在酵母提取物中鉴定出的该基因产物含有核心大小的碳水化合物。MPD1对生长不是必需的，但该基因的过表达抑制了由PDI1缺失引起的羧肽酶Y的成熟缺陷，表明其在酵母内质网中与PDI具有相关功能。
