Gavériaux C, Peluso J, Simonin F, Laforet J, Kieffer B
Ecole Supérieure de Biotechnologie de Strasbourg, Illkirch, France.
FEBS Lett. 1995 Aug 7;369(2-3):272-6. doi: 10.1016/0014-5793(95)00766-3.
To investigate the role of opioids as direct modulators of the immune response, we have searched for expression of the recently cloned delta, mu and kappa opioid receptors in immune cells. We have devised a reverse transcriptase-polymerase chain reaction strategy which specifically detects a region spanning putative transmembrane regions 2 to 7 for each transcript in both human and mouse immune cells. In human peripheral blood lymphocyte and monocyte preparations, delta was undetectable while the kappa transcript was present. The analysis of human cell lines revealed low but significant levels of delta opioid receptor transcripts in T, B or monocyte cell lines while the kappa transcript was found in B cell lines only. Investigation of murine cells showed the presence of transcript for the delta receptor in splenocytes and in some T and B cell lines. Unexpectedly, no expression of the mu receptor was detected. Sequence analysis of PCR products demonstrated nucleotide identity between immune and neuronal transcripts, indicating that they derive from the same genes. In conclusion, our results lead to the identification of kappa and delta opioid receptor transcripts in immune cells.
为了研究阿片类药物作为免疫反应直接调节因子的作用，我们搜索了免疫细胞中最近克隆的δ、μ和κ阿片受体的表达情况。我们设计了一种逆转录聚合酶链反应策略，该策略可特异性检测人和小鼠免疫细胞中每个转录本跨越假定跨膜区域2至7的区域。在人外周血淋巴细胞和单核细胞制剂中，未检测到δ，而存在κ转录本。对人细胞系的分析显示，在T、B或单核细胞系中，δ阿片受体转录本水平较低但显著，而κ转录本仅在B细胞系中发现。对鼠细胞的研究表明，脾细胞以及一些T和B细胞系中存在δ受体的转录本。出乎意料的是，未检测到μ受体的表达。PCR产物的序列分析表明免疫和神经元转录本之间存在核苷酸同一性，表明它们来自相同的基因。总之，我们的结果导致在免疫细胞中鉴定出κ和δ阿片受体转录本。
