Ferris S, Watson H L, Neyrolles O, Montagnier L, Blanchard A
Institut Pasteur, Departement du SIDA et des Retrovirus, Unité d'Oncologie Virale et URA CNRS 1157, Paris, France.
FEMS Microbiol Lett. 1995 Aug 1;130(2-3):313-9. doi: 10.1111/j.1574-6968.1995.tb07737.x.
A novel mycoplasmal species designated as Mycoplasma penetrans has been isolated recently from patients infected with human immunodeficiency virus. p35, a major antigen extracted from the membrane of this mycoplasma using Triton X-114 has been found to be a lipoprotein. After proteolytic treatment of p35, the sequence of one of the resulting peptides was determined and a corresponding oligonucleotide was deduced. Using this oligonucleotide as a probe the p35 gene was cloned and sequenced. Sequence analysis revealed an amino-terminal signal peptide with a potential acylation site which would result in a 35.3 kDa mature product. In addition, the p35 gene was followed by an open reading frame with a corresponding polypeptide partially homologous to p35, in particular to the N-terminus region.
最近，从感染人类免疫缺陷病毒的患者中分离出了一种新的支原体物种，命名为穿透支原体。使用Triton X-114从这种支原体膜中提取的主要抗原p35已被发现是一种脂蛋白。对p35进行蛋白水解处理后，确定了其中一个所得肽段的序列，并推导了相应的寡核苷酸。使用该寡核苷酸作为探针，克隆并测序了p35基因。序列分析揭示了一个带有潜在酰化位点的氨基末端信号肽，这将产生一个35.3 kDa的成熟产物。此外，p35基因后面是一个开放阅读框，其相应的多肽与p35部分同源，特别是与N末端区域同源。
