D'Andrea A, Chang C, Franz-Bacon K, McClanahan T, Phillips J H, Lanier L L
Department of Human Immunology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304, USA.
J Immunol. 1995 Sep 1;155(5):2306-10.
The expression of certain MHC class I allotypes by potential target cells can inhibit NK cell-mediated cytotoxicity. We recently identified the NKB1 surface Ag, expressed on T and NK cell subsets, as a putative inhibitory receptor for HLA-B class I molecules possessing the Bw4 serologic epitope. NKB1 is a 70-kDa glycoprotein that after deglycosylation migrates as a 50-kDa protein as determined by SDS-PAGE. A cDNA encoding the NKB1 receptor was cloned from a NKB1+T cell cDNA library by expression in COS-7 cells using the anti-NKB1 mAb DX9. NKB1 is a member of the lg superfamily containing three lg-like domains in the extracellular region and is related to the recently identified family (p58/NKAT) of human NK and T cell surface molecules that appear to function as inhibitory receptors for HLA class I.
潜在靶细胞表达的某些I类主要组织相容性复合体（MHC）同种异型可抑制自然杀伤（NK）细胞介导的细胞毒性。我们最近鉴定出NKB1表面抗原，它表达于T细胞和NK细胞亚群，是具有Bw4血清学表位的HLA - B I类分子的一种假定抑制性受体。NKB1是一种70 kDa的糖蛋白，经去糖基化后，通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）测定，迁移为50 kDa的蛋白。使用抗NKB1单克隆抗体DX9，通过在COS - 7细胞中表达，从NKB1 + T细胞cDNA文库中克隆出编码NKB1受体的cDNA。NKB1是免疫球蛋白（Ig）超家族的成员，在细胞外区域含有三个Ig样结构域，并且与最近鉴定出的人类NK和T细胞表面分子家族（p58 / NKAT）相关，这些分子似乎作为HLA I类的抑制性受体发挥作用。
