Howell C D, Li J, Roper E, Kotzin B L
Department of Medicine, University of Colorado School of Medicine, Denver 80262, USA.
J Immunol. 1995 Sep 1;155(5):2350-8.
Murine graft-vs-host disease (GVHD) results in destruction of small bile ducts in the liver. We analyzed the TCR V beta repertoire of lymphocytes isolated from the livers and spleens of individual B10.D2 into irradiated BALB/c GVHD mice by means of two-color immunofluorescence. Each mouse showed an increase in at least one V beta population in the liver and spleen, but the expanded V beta populations were heterogeneous and variable among individual GVHD mice. Overall, the repertoire of liver CD4 cells was biased toward V beta 2 and 3 expression with 65 and 88% of mice, respectively, showing an increase in these subsets. The splenic CD4 cell repertoire was biased toward V beta 3 and 4 expression (50% of mice each). The repertoire of CD8 cells was less biased with 20 to 35% of mice showing expansions of V beta 3+, 4+, 5+, 6+, 8.1+, 8.2+, and 8.3+ T cells in both the liver and spleen. V beta 2+ CD4 cells were increased preferentially in the liver compared with the spleen. These results indicate that the infiltrating liver and splenic T cells are polyclonal and suggest that donor T cells recognize multiple host non-MHC Ags in this GVHD model. Alloantigens recognized by V beta 2+ CD4 cells appear to be selective for the liver. Expansion of V beta 3+ CD4 cells may reflect recognition of the host Mls-3 superantigen.
小鼠移植物抗宿主病（GVHD）会导致肝脏中小胆管的破坏。我们通过双色免疫荧光法分析了从个体B10.D2的肝脏和脾脏中分离出来的淋巴细胞的TCR Vβ谱，这些淋巴细胞被注入受辐照的BALB/c GVHD小鼠体内。每只小鼠肝脏和脾脏中至少有一个Vβ群体增加，但在各个GVHD小鼠中，扩增的Vβ群体是异质性的且各不相同。总体而言，肝脏CD4细胞的谱型偏向于Vβ2和Vβ3表达，分别有65%和88%的小鼠显示这些亚群增加。脾脏CD4细胞的谱型偏向于Vβ3和Vβ4表达（各有50%的小鼠）。CD8细胞的谱型偏向性较小，20%至35%的小鼠在肝脏和脾脏中均显示Vβ3 +、Vβ4 +、Vβ5 +、Vβ6 +、Vβ8.1 +、Vβ8.2 +和Vβ8.3 + T细胞扩增。与脾脏相比，Vβ2 + CD4细胞在肝脏中优先增加。这些结果表明，浸润肝脏和脾脏的T细胞是多克隆的，提示在该GVHD模型中供体T细胞识别多种宿主非MHC抗原。Vβ2 + CD4细胞识别的同种抗原似乎对肝脏具有选择性。Vβ3 + CD4细胞的扩增可能反映了对宿主Mls - 3超抗原的识别。
