新生蛋白质在无细胞体系中加工成糖基磷脂酰肌醇锚定形式。 - Suppr

Processing of nascent proteins to glycosylphosphatidylinositol-anchored forms in cell-free systems.

作者信息

Kodukula K, Maxwell S E, Udenfriend S

机构信息

Department of Natural Products Research, Bristol-Myers Squibb Pharmaceutical Research Institute, Wallingford, Connecticut 06492, USA.

出版信息

Methods Enzymol. 1995;250:536-47. doi: 10.1016/0076-6879(95)50095-2.

DOI:10.1016/0076-6879(95)50095-2

PMID:7651176

Abstract

摘要

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Processing of nascent proteins to glycosylphosphatidylinositol-anchored forms in cell-free systems.新生蛋白质在无细胞体系中加工成糖基磷脂酰肌醇锚定形式。

Methods Enzymol. 1995;250:536-47. doi: 10.1016/0076-6879(95)50095-2.

Biosynthesis of glycosylphosphatidylinositol (GPI)-anchored membrane proteins in intact cells: specific amino acid requirements adjacent to the site of cleavage and GPI attachment.完整细胞中糖基磷脂酰肌醇（GPI）锚定膜蛋白的生物合成：切割和GPI连接位点附近的特定氨基酸需求。

J Cell Biol. 1993 Feb;120(3):657-64. doi: 10.1083/jcb.120.3.657.

Cleavage without anchor addition accompanies the processing of a nascent protein to its glycosylphosphatidylinositol-anchored form.在新生蛋白质加工成糖基磷脂酰肌醇锚定形式的过程中会伴随无锚定添加的切割。

Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1550-4. doi: 10.1073/pnas.92.5.1550.

Conversion of secretory proteins into membrane proteins by fusing with a glycosylphosphatidylinositol anchor signal of alkaline phosphatase.通过与碱性磷酸酶的糖基磷脂酰肌醇锚定信号融合，将分泌蛋白转化为膜蛋白。

Biochem J. 1994 Jul 15;301 ( Pt 2)(Pt 2):577-83. doi: 10.1042/bj3010577.

Prediction of omega site in nascent precursor of glycosylphosphatidylinositol protein.糖基磷脂酰肌醇蛋白新生前体中ω位点的预测

Methods Enzymol. 1995;250:571-82. doi: 10.1016/0076-6879(95)50098-7.

Biosynthesis of phosphatidylinositol glycan-anchored membrane proteins. Design of a simple protein substrate to characterize the enzyme that cleaves the COOH-terminal signal peptide.磷脂酰肌醇聚糖锚定膜蛋白的生物合成。用于鉴定切割COOH末端信号肽的酶的简单蛋白质底物的设计。

J Biol Chem. 1991 Mar 5;266(7):4464-70.

Phosphatidylinositol-glycan (PI-G)-anchored membrane proteins: requirement of ATP and GTP for translation-independent COOH-terminal processing.磷脂酰肌醇聚糖（PI-G）锚定膜蛋白：翻译非依赖性COOH末端加工对ATP和GTP的需求

Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):6124-8. doi: 10.1073/pnas.89.13.6124.

Placental alkaline phosphatase: a model for studying COOH-terminal processing of phosphatidylinositol-glycan-anchored membrane proteins.胎盘碱性磷酸酶：研究磷脂酰肌醇聚糖锚定膜蛋白羧基末端加工的模型。

Clin Chem. 1992 Dec;38(12):2510-6.

Selectivity at the cleavage/attachment site of phosphatidylinositol-glycan anchored membrane proteins is enzymatically determined.磷脂酰肌醇聚糖锚定膜蛋白切割/附着位点的选择性由酶促决定。

Proc Natl Acad Sci U S A. 1990 Oct;87(20):7939-43. doi: 10.1073/pnas.87.20.7939.

Cell-free processing of nascent proteins destined to be linked to the plasma membrane by a phosphatidylinositol-glycan anchor.通过磷脂酰肌醇聚糖锚定与质膜相连的新生蛋白质的无细胞加工。

Cell Mol Biol. 1992 Feb;38(1):11-6.

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Darkness in the Human Gene and Protein Function Space: Widely Modest or Absent Illumination by the Life Science Literature and the Trend for Fewer Protein Function Discoveries Since 2000.人类基因和蛋白质功能领域的黑暗：生命科学文献的光照范围普遍较窄或不存在，以及自 2000 年以来蛋白质功能发现数量减少的趋势。

Proteomics. 2018 Nov;18(21-22):e1800093. doi: 10.1002/pmic.201800093. Epub 2018 Oct 30.

Transamidase subunit GAA1/GPAA1 is a M28 family metallo-peptide-synthetase that catalyzes the peptide bond formation between the substrate protein's omega-site and the GPI lipid anchor's phosphoethanolamine.转酰胺酶亚基GAA1/GPAA1是一种M28家族金属肽合成酶，可催化底物蛋白ω位点与GPI脂质锚定物磷酸乙醇胺之间的肽键形成。

Cell Cycle. 2014;13(12):1912-7. doi: 10.4161/cc.28761. Epub 2014 Apr 17.

Glycosyl-phosphatidylinositol anchor attachment in a yeast in vitro system.酵母体外系统中的糖基磷脂酰肌醇锚定连接

Biochem J. 1997 Dec 1;328 ( Pt 2)(Pt 2):669-75. doi: 10.1042/bj3280669.

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Processing of nascent proteins to glycosylphosphatidylinositol-anchored forms in cell-free systems.

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