促黄体生成激素释放激素（LRH）C端和N端特异性放射免疫测定
C- and N-terminal specific LH-releasing hormone (LRH) radioimmunoassay.
作者信息
Dahlén H G, Voigt K H, Schneider H P
出版信息
Horm Metab Res. 1976 Jan;8(1):61-6. doi: 10.1055/s-0028-1093674.
A highly sensitive and specific LRH radioimmunoassay is described. Three antisera were compared and characterized. High specificity was shown by 2 of them in that both C- and N-terminals were required for binding when testing LRH analogs. A lower detection limit of about 1 pg could be obtained using a simple organic solvent precipitation method for separation of bound/free antigen. Good correlation with the Ramirez-McCann bioassay was obtained, and rat median eminence extracts contained a material immunologically similar to synthetic LRH as indicated by parallel inhibition curves. Direct measurement of radioimmunoassayable LRH in plasma can lead to misleading results due to nonspecific interference of plasma factors. After employing a simple deproteinization method, rat plasma was found to contain less than 1-3 pg LRH/ml. Rat median eminence extracts contained approx. 7 ng LRH.
本文描述了一种高灵敏度和特异性的促黄体激素释放激素（LRH）放射免疫分析方法。对三种抗血清进行了比较和特性鉴定。其中两种抗血清表现出高特异性，即在检测LRH类似物时，C末端和N末端都需要结合。使用简单的有机溶剂沉淀法分离结合/游离抗原，可获得约1皮克的较低检测限。与拉米雷斯 - 麦肯生物测定法具有良好的相关性，并且大鼠正中隆起提取物含有一种免疫上与合成LRH相似的物质，平行抑制曲线表明了这一点。由于血浆因子的非特异性干扰，直接测量血浆中可进行放射免疫分析的LRH可能会导致误导性结果。采用简单的脱蛋白方法后，发现大鼠血浆中LRH含量低于1 - 3皮克/毫升。大鼠正中隆起提取物含有约7纳克LRH。
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