蜱传和昆虫传环状病毒非结构蛋白NS1的比较

Comparison of the non-structural protein, NS1, of tick-borne and insect-borne orbiviruses.

作者信息

Moss S R, Nuttall P A

机构信息

NERC Institute of Virology & Environmental Microbiology, Oxford, UK.

出版信息

Virus Res. 1995 May;36(2-3):287-92. doi: 10.1016/0168-1702(95)00004-a.

DOI:10.1016/0168-1702(95)00004-a

PMID:7653106

Abstract

The nucleotide sequence of RNA segment 6 of Broadhaven virus (BRDV), a tick-borne orbivirus, was determined principally from two overlapping cDNA clones and RNA end sequence analysis. The genome segment is 1714 base pairs in length and has a coding capacity for a protein of 537 amino acids, having a net charge of +4.0 at neutral pH. Comparison of the predicted amino acid sequence of BRDV RNA segment 6 with the NS1 sequence of insect-borne orbiviruses, bluetongue virus (BTV), African horse sickness virus (AHSV) and epizootic haemorrhagic disease virus (EHDV) of deer, revealed amino acid identities of 21, 22 and 21%, respectively. This compares with amino acid identities of 31 to 50% between the NS1 proteins of these gnat-transmitted orbiviruses. A recombinant baculovirus was produced containing a full-length clone of BRDV segment 6, which expressed a protein of 61 kD in infected Spodoptera frugiperda cells. Like other orbivirus NS1 proteins the expressed protein formed tubules similar to those produced in BRDV-infected BHK21 cells.

摘要

布罗德黑文病毒（BRDV）是一种蜱传环状病毒，其RNA片段6的核苷酸序列主要通过两个重叠的cDNA克隆和RNA末端序列分析来确定。该基因组片段长度为1714个碱基对，编码一种由537个氨基酸组成的蛋白质，在中性pH值下净电荷为+4.0。将BRDV RNA片段6的预测氨基酸序列与昆虫传播的环状病毒蓝舌病毒（BTV）、非洲马瘟病毒（AHSV）以及鹿的流行性出血病病毒（EHDV）的NS1序列进行比较，结果显示氨基酸同源性分别为21%、22%和21%。相比之下，这些蚋传播的环状病毒的NS1蛋白之间的氨基酸同源性为31%至50%。构建了一种重组杆状病毒，其中包含BRDV片段6的全长克隆，该克隆在感染的草地贪夜蛾细胞中表达了一种61 kD的蛋白质。与其他环状病毒的NS1蛋白一样，表达的蛋白质形成了类似于在BRDV感染的BHK21细胞中产生的小管。