Nordstoga K, Zhou Z Y, Husby G
Department of Morphology, Genetics and Aquatic Biology, Norwegian College of Veterinary Medicine, Oslo.
Zentralbl Veterinarmed A. 1994 Dec;41(10):741-7. doi: 10.1111/j.1439-0442.1994.tb00144.x.
Bovine kidney material with advanced glomerular deposits of amyloid was studied immunohistochemically using the avidin biotin complex immunoperoxidase method, with rabbit anti-horse AA serum as primary antibody. Severely affected glomeruli consisted of strongly reacting positive material, obscuring all cellular structures. Transmission electron microscopy revealed that the amyloidotic areas, with evident amyloid fibrils, also contained a considerable admixture of cellular remnants. In this investigation it was found that such material was more abundant in the bovine glomerular amyloid masses than in amyloid laden organs from other animal species, and it is discussed to what extent this observation may explain the varying tinctorial properties of amyloid deposits in bovine tissues, and the relative high content of histones in bovine amyloid proteins.
使用抗生物素蛋白-生物素复合物免疫过氧化物酶方法，以兔抗马AA血清作为一抗，对具有晚期肾小球淀粉样沉积物的牛肾材料进行免疫组织化学研究。严重受累的肾小球由强反应阳性物质组成，使所有细胞结构模糊不清。透射电子显微镜显示，具有明显淀粉样纤维的淀粉样变区域还含有相当数量的细胞残余物混合物。在本研究中发现，这种物质在牛肾小球淀粉样肿块中比在其他动物物种的淀粉样负荷器官中更丰富，并讨论了这一观察结果在多大程度上可以解释牛组织中淀粉样沉积物不同的染色特性，以及牛淀粉样蛋白中组蛋白的相对高含量。
