Hiszczyńska-Sawicka E, Kur J
Department of Microbiology, Technical University of Gdańsk, Poland.
Acta Biochim Pol. 1995;42(1):103-8.
The integration host factor (IHF) is a sequence-specific, histone-like, multi-functional DNA-binding and -bending protein of Escherichia coli. Characterization and functional analysis of this protein has been carried out mainly in bacteriophage lambda and other mobile genetic elements. In this paper we report data concerning the binding of IHF protein to the plasmid orip15A region. IHF binds to the single site of the DNA fragment containing the orip15A, as shown by the gel mobility shift assays and footprinting experiment. On the basis of the ihf consensus sequences published, we have been able to identify one sequence of putative ihf site into the orip15A sequence with two mismatches in relation to the consensus sequence of Kur et al., 1989, Gene 81, 1-15. One ihf binding site was also found in the oriColE1 region sequence with three mismatches in relation to this consensus sequence.
整合宿主因子（IHF）是一种序列特异性、组蛋白样、多功能的大肠杆菌DNA结合和弯曲蛋白。对该蛋白的表征和功能分析主要在噬菌体λ和其他可移动遗传元件中进行。在本文中，我们报告了有关IHF蛋白与质粒ori15A区域结合的数据。凝胶迁移率变动分析和足迹实验表明，IHF与包含ori15A的DNA片段的单个位点结合。根据已发表的ihf共有序列，我们能够在ori15A序列中鉴定出一个推定的ihf位点序列，与Kur等人1989年发表在《基因》第81卷第1 - 15页的共有序列相比有两个错配。在oriColE1区域序列中也发现了一个ihf结合位点，与该共有序列相比有三个错配。
