西葫芦胰蛋白酶抑制剂编码基因的合成、克隆及在大肠杆菌中的表达
Synthesis, cloning and expression in Escherichia coli of the gene coding for the trypsin inhibitor from Cucurbita pepo.
作者信息
Rempoøa B, Wilusz T, Markiewicz W, Fikus M
机构信息
Institute of Biochemistry and Biophysics of the Polish Academy of Sciences, Warsaw.
出版信息
Acta Biochim Pol. 1995;42(1):109-14.
PMID:7653151
Abstract
A chemically synthesized gene coding for the serine proteinase inhibitor CPTI II was cloned in E. coli and its expression was investigated in cytoplasmic and secretion systems. Under all conditions investigated the biologically active form of the inhibitor was found only in the latter system, although the yield was rather low.
摘要
编码丝氨酸蛋白酶抑制剂CPTI II的化学合成基因在大肠杆菌中克隆，并在细胞质和分泌系统中研究其表达。在所研究的所有条件下，尽管产量相当低，但仅在后一种系统中发现了抑制剂的生物活性形式。
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