Krotkiewski H, Duk M, Lisowska E
Department of Immunochemistry, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.
Acta Biochim Pol. 1995;42(1):41-4.
A higher content of Tn and sialyl-Tn receptors in glycophorin A of blood group N than in that of blood group M was suggested by reactions with anti-Tn lectins. Analysis of beta-elimination products of two blood group M and two blood group N preparations by gas liquid chromatography-mass spectrometry showed that GalNAc-ol was detectable in minor amounts in all analyzed samples and its content was higher in the products obtained from desialylated antigens. Moreover, the content of GalNAc-ol detected in blood group N samples was almost twice as high as in respective blood group M samples. Since blood group M and N antigens differ in two amino-acid residues, our results support the existence of sequence-dependent differences in efficiency of substitution of glycophorin GalNAc-Ser/Thr residues with galactose.
抗Tn凝集素反应表明，血型N的血型糖蛋白A中Tn和唾液酸化Tn受体的含量高于血型M。通过气相色谱-质谱联用对两种血型M和两种血型N制剂的β-消除产物进行分析，结果显示，在所有分析样品中均能检测到少量的N-乙酰半乳糖胺醇（GalNAc-ol），且其含量在去唾液酸化抗原的产物中更高。此外，血型N样品中检测到的GalNAc-ol含量几乎是相应血型M样品的两倍。由于血型M和N抗原在两个氨基酸残基上存在差异，我们的结果支持血型糖蛋白GalNAc-Ser/Thr残基被半乳糖取代的效率存在序列依赖性差异。
