Wypijewski K J, Musiaø W G, Misztal L, Pater B, Malinowski T, Augustyniak J
A. Mickiewicz University, Department of Biopolymer Biochemistry, Poznań, Poland.
Acta Biochim Pol. 1995;42(1):97-101.
Plant expression vector pBI 121 containing the gene encoding coat protein of Plum Pox Virus of the Skierniewice isolate (CP PPV-S) was prepared (clone pCM1). The construct was used for transformation of Nicotiana tabacum plants using an Agrobacterium tumefaciens based system. About 82% of kanamycin resistant plant lines contained a transgene (the sequence of CP PPV-S) but only 81% of them actively expressed the PPV-S coat protein gene as measured by RT-PCR.
制备了含有斯基尔尼维采分离株李痘病毒外壳蛋白编码基因（CP PPV-S）的植物表达载体pBI 121（克隆pCM1）。使用基于根癌农杆菌的系统，将该构建体用于烟草植物的转化。约82%的卡那霉素抗性植物株系含有转基因（CP PPV-S序列），但通过逆转录聚合酶链反应检测，其中只有81%的株系能有效表达PPV-S外壳蛋白基因。
