Hansz J, Kozłowska-Skrzypczak M, Sawiński K, Włodarczyk M
Kliniki Hematologii AM w Poznaniu.
Acta Haematol Pol. 1995;26(2):185-93.
The effect of peripheral blood T lymphocytes from 42 patients with advanced Hodgkin's disease (grade III and IV) on the autologous marrow erythroid colony formation was studied in diffusion chamber culture. It was found, that unfractionated T lymphocytes suppress the BFU-E--(burst forming unit erythroid) and CFU-E--(colony forming unit erythroid)--derived colony formation by releasing an inhibitory activity. The suppression of colony formation was noted already at 0.25 x 10(5) cell concentration. In experiments with 0.5 x 10(5) and 1.0 x 10(5) T cells the inhibitory effect was increased. Subsequently it was shown, that this inhibition was generated by radioresistant, CD8+ and HLA-DR- subset of T cells. In control experiments, T lymphocytes from healthy subjects had no influence on the erythroid colony formation.
在扩散室培养中研究了42例晚期霍奇金病（III级和IV级）患者外周血T淋巴细胞对自体骨髓红系集落形成的影响。发现未分离的T淋巴细胞通过释放抑制活性来抑制BFU-E（红系爆式集落形成单位）和CFU-E（红系集落形成单位）衍生的集落形成。在0.25×10⁵细胞浓度时就已观察到集落形成受到抑制。在使用0.5×10⁵和1.0×10⁵T细胞的实验中，抑制作用增强。随后表明，这种抑制是由T细胞的放射抗性、CD8⁺和HLA-DR⁻亚群产生的。在对照实验中，健康受试者的T淋巴细胞对红系集落形成没有影响。
