Woods E, Ohashi T, Polk D, Ikegami M, Ueda T, Jobe A H
Department of Obstetrics and Gynecology, Harbor-University of California Los Angeles Medical Center, Torrance 90509, USA.
Am J Physiol. 1995 Aug;269(2 Pt 1):L209-14. doi: 10.1152/ajplung.1995.269.2.L209.
The effects of exogenous surfactant treatment combined with postnatal ventilation on surfactant protein (SP)-A, SP-B, and SP-C steady-state mRNA levels were evaluated in preterm sheep at 120, 132, and 139 days gestation. Three groups were studied at each gestation period: animals ventilated and treated with 100 mg/kg natural sheep surfactant, animals ventilated and not treated with surfactant, and a comparison group of lambs that were neither ventilated nor treated with surfactant. In unventilated lambs, SP-A and SP-C mRNA levels measured by densitometry from Northern blots increased between 120 and 132 days gestation (P < 0.05). At 120 days gestation, no differences in SP-A, SP-B, or SP-C mRNA levels were noted among the three groups. At 132 days gestation, SP-A mRNA levels increased in both ventilated groups (P < 0.01), but no additional surfactant effect on SP-A mRNA expression was detected. There were no changes in SP-B or SP-C mRNA levels among the groups at 132 days gestation. At 139 days gestation, mRNA levels for both SP-A and SP-B increased after ventilation, compared with the unventilated groups (P < 0.05). Furthermore, an additional effect of surfactant treatment to increase SP-A mRNA levels relative to animals undergoing ventilation alone was noted (P < 0.05). We conclude that postnatal changes in mRNA levels for the surfactant proteins are gestationally regulated and protein specific.
在妊娠120、132和139天的早产绵羊中，评估了外源性表面活性剂治疗联合产后通气对表面活性蛋白（SP）-A、SP-B和SP-C稳态mRNA水平的影响。在每个妊娠期研究了三组：用100mg/kg天然绵羊表面活性剂通气并治疗的动物、通气但未用表面活性剂治疗的动物，以及既未通气也未用表面活性剂治疗的羔羊对照组。在未通气的羔羊中，通过Northern印迹光密度法测量的SP-A和SP-C mRNA水平在妊娠120至132天之间升高（P<0.05）。在妊娠120天时，三组之间的SP-A、SP-B或SP-C mRNA水平没有差异。在妊娠132天时，两个通气组的SP-A mRNA水平均升高（P<0.01），但未检测到表面活性剂对SP-A mRNA表达的额外影响。在妊娠132天时，各组之间的SP-B或SP-C mRNA水平没有变化。在妊娠139天时，与未通气组相比，通气后SP-A和SP-B的mRNA水平均升高（P<0.05）。此外，还注意到表面活性剂治疗相对于仅接受通气的动物有额外增加SP-A mRNA水平的作用（P<0.05）。我们得出结论，表面活性蛋白mRNA水平的产后变化受妊娠期调节且具有蛋白特异性。
