Roncin S, Guillevin L, Beaugrand M, Vallet S, Pennec Y L, Colin J, Youinou P
Service d'Ophtalmologie, CHU, Brest.
Ann Med Interne (Paris). 1995;146(4):226-8.
T lymphocytes are predominantly involved in the development of Sjögren's syndrome. Their repertoire has recently been claimed to be restricted. Analysis of T cell receptor V beta gene products within the labial salivary glands led us to identify V beta 2 and V beta 8. This study, using a new panel of anti-V beta product monoclonal antibodies and a tissue triple-staining technique, examined the distribution of V beta gene family products in activated as well as non-activated CD4+ or CD8+ T cells subsets in the salivary gland tissue of SS patients. Our results suggest that the V beta genes used by the T CD4+ or T CD8+ cells in situ are similar, irrespective of the activation status of these cells. T lymphocytes homing into exocrine tissues might thus be selected on the basis of their V beta repertoire rather than their activation state.
T淋巴细胞在干燥综合征的发病过程中起主要作用。最近有人声称其受体库受到限制。对唇腺内T细胞受体Vβ基因产物的分析使我们鉴定出了Vβ2和Vβ8。本研究使用一组新的抗Vβ产物单克隆抗体和组织三重染色技术，检测了Vβ基因家族产物在干燥综合征患者唾液腺组织中活化及未活化的CD4+或CD8+ T细胞亚群中的分布。我们的结果表明，原位CD4+或CD8+ T细胞所使用的Vβ基因相似，与这些细胞的活化状态无关。因此，归巢至外分泌组织的T淋巴细胞可能是根据其Vβ受体库而非其活化状态来选择的。
