Ebuchi M, Sakamoto S, Kudo H, Nagase J, Endo M
Department of Surgery, Tokyo Metropolitan Ookubo Hospital, Japan.
Anticancer Res. 1995 Jul-Aug;15(4):1481-4.
The object of this study was to evaluate activities of thymidylate synthetase (TS) and thymidine kinase (TK) in relation to histopathological features of human mammary carcinomas, as well as clinical classification of patients. The TS and TK are key enzymes on the de novo and salvage pathways, respectively, for pyrimidine nucleotide synthesis. High TS and TK activities have been found in rapidly proliferating tissues. Clinical stage and TNM post-surgical histopathological classification of 20 patients with mammary carcinomas, during the period of 1988-1991, and TS and TK activities in each carcinoma were determined. Elevated TS and TK activities in mammary carcinomas corresponded to progressively worse clinical stages. Papillotubular carcinomas showed lower enzyme activity than that of solid-tubular and scirrhous types. TS activities continued to rise as the malignant invasion became more severe. The clinical prognosis of patients with mammary carcinomas appears to depend on the de novo synthesis of DNA in carcinomas.
本研究的目的是评估胸苷酸合成酶（TS）和胸苷激酶（TK）的活性与人类乳腺癌组织病理学特征以及患者临床分类之间的关系。TS和TK分别是嘧啶核苷酸从头合成途径和补救途径中的关键酶。在快速增殖的组织中发现TS和TK活性较高。测定了1988年至1991年期间20例乳腺癌患者的临床分期和术后TNM组织病理学分类，以及每例癌组织中的TS和TK活性。乳腺癌中TS和TK活性升高与临床分期逐渐恶化相对应。乳头状管状癌的酶活性低于实体管状癌和硬癌类型。随着恶性浸润加重，TS活性持续升高。乳腺癌患者的临床预后似乎取决于癌组织中DNA的从头合成。
