脊椎动物芳烃（二噁英）受体的进化保守性：硬骨鱼芳烃受体cDNA PAS结构域的扩增与测序
Evolutionary conservation of the vertebrate Ah (dioxin) receptor: amplification and sequencing of the PAS domain of a teleost Ah receptor cDNA.
作者信息
Hahn M E, Karchner S I
机构信息
Biology Department, Woods Hole Oceanographic Institution, MA 02543-1049, USA.
出版信息
Biochem J. 1995 Sep 1;310 ( Pt 2)(Pt 2):383-7. doi: 10.1042/bj3100383.
Abstract
The PAS domain of a teleost Ah receptor was amplified using reverse transcription-PCR with degenerate primers containing inosine. The deduced amino acid sequence of the amplified cDNA fragment was 62-64% identical with the PAS domains of mammalian Ah receptors. These data demonstrate the homology of Ah receptors in mammals and fish, and reveal regions of this protein that are highly conserved between these diverse vertebrate groups.
摘要
使用含有次黄苷的简并引物通过逆转录聚合酶链反应扩增了硬骨鱼芳烃受体的PAS结构域。扩增的cDNA片段推导的氨基酸序列与哺乳动物芳烃受体的PAS结构域具有62%-64%的同一性。这些数据证明了哺乳动物和鱼类中芳烃受体的同源性，并揭示了在这些不同脊椎动物类群之间该蛋白质高度保守的区域。
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