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大鼠核糖体蛋白的一级结构:S21和L39的cDNA特性、L7和L18a序列的校正以及L33的鉴定

The primary structures of rat ribosomal proteins: the characterization of the cDNAs for S21 and L39, corrections in the sequences of L7 and L18a, and the identification of L33.

作者信息

Chan Y L, Olvera J, Wool I G

机构信息

Department of Biochemistry and Molecular Biology, University of Chicago, Illinois 60637, USA.

出版信息

Biochem Biophys Res Commun. 1995 Aug 24;213(3):1042-50. doi: 10.1006/bbrc.1995.2233.

Abstract

cDNAs for rat ribosomal proteins S21 and L39 were characterized. S21 has 83 amino acids and a molecular weight of 9,121; L39 has 50 amino acids and a molecular weight of 6,271. There are 8 to 9 copies of the S21, and 9 to 11 copies of the L39 genes. The mRNA for S21 is about 500 nucleotides in length and for L39 it is about 450. Rat S21 and L39 are related to ribosomal proteins from other species. Corrections have been made in the amino acid sequences of L7 and L18a. The rat ribosomal protein designated L33 from its coordinates on two-dimensional gels has been found, from its amino acid sequence, to be identical to S24.

摘要

对大鼠核糖体蛋白S21和L39的cDNA进行了表征。S21含有83个氨基酸,分子量为9121;L39含有50个氨基酸,分子量为6271。S21基因有8至9个拷贝,L39基因有9至11个拷贝。S21的mRNA长度约为500个核苷酸,L39的mRNA长度约为450个核苷酸。大鼠的S21和L39与其他物种的核糖体蛋白相关。已对L7和L18a的氨基酸序列进行了校正。从二维凝胶上的坐标确定的大鼠核糖体蛋白L33,根据其氨基酸序列,发现与S24相同。

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