Kelly J F, Mason R P, Denisova N A, Joseph J A, Erat S, Roth G S
Molecular Physiology and Genetics Section, National Institute on Aging, Baltimore, MD 21224, USA.
Biochem Biophys Res Commun. 1995 Aug 24;213(3):869-74. doi: 10.1006/bbrc.1995.2209.
In order to determine whether age-related changes in neuronal membrane structure contribute to previously reported changes in muscarinic cholinergic signal transduction, striata from 3, 13 and 23 month old F344 male rats were examined for both carbachol-stimulated low Km GTPase activity and membrane one-dimensional electron density profile using small angle X-ray diffraction. Increasing age was associated with both a reduction in stimulated GTPase activity and a decrease in membrane bilayer width. These findings suggest the possibility that fundamental membrane structural changes may contribute to alterations in signal transduction seen with aging.
为了确定神经元膜结构的年龄相关性变化是否导致先前报道的毒蕈碱胆碱能信号转导变化，使用小角X射线衍射检查了3、13和23月龄F344雄性大鼠的纹状体，以检测卡巴胆碱刺激的低Km GTP酶活性和膜一维电子密度分布。年龄增长与刺激的GTP酶活性降低和膜双层宽度减小有关。这些发现提示了一种可能性，即基本的膜结构变化可能导致衰老过程中所见的信号转导改变。
