Zhao T J, Chiu T H, Rosenberg H C
Department of Pharmacology, Medical College of Ohio, Toledo 43699, USA.
J Mol Neurosci. 1994 Fall;5(3):181-92. doi: 10.1007/BF02736732.
The expression of GABAA/benzodiazepine beta subunit mRNAs was studied in cerebral cortex, hippocampus, and cerebellum of flurazepam-treated rats. Immediately following 4 wk of treatment, beta 2 and beta 3 subunit mRNAs were significantly reduced in cerebellum and hippocampus, whereas only beta 2 was decreased in cortex. These decreases had largely reversed 48 h following flurazepam treatment. After 2 wk of treatment, both beta 2 and beta 3 mRNAs were reduced in cerebellum, and beta 3 mRNA was reduced in hippocampus, but neither was changed in cortex. Four hours after an acute flurazepam treatment, the only change was a decrease in beta 3 mRNA in hippocampus. These results indicate that the expression of GABAA receptor beta subunit mRNAs in different brain regions is differentially regulated during chronic flurazepam treatment, and some changes occur within hours after a single large dose.
在接受氟西泮治疗的大鼠的大脑皮层、海马体和小脑中研究了GABAA/苯二氮䓬β亚基mRNA的表达。在治疗4周后立即检测发现，小脑和海马体中的β2和β3亚基mRNA显著减少，而皮层中只有β2减少。在氟西泮治疗48小时后，这些减少大多逆转。治疗2周后，小脑中β2和β3 mRNA均减少，海马体中β3 mRNA减少，但皮层中两者均未改变。急性氟西泮治疗4小时后，唯一的变化是海马体中β3 mRNA减少。这些结果表明，在慢性氟西泮治疗期间，不同脑区的GABAA受体β亚基mRNA的表达受到不同的调节，并且在单次大剂量给药后的数小时内会发生一些变化。
