Hilario E, Gogarten J P
Department of Molecular and Cell Biology, University of Connecticut, Storrs 06269-3044, USA.
Biochim Biophys Acta. 1995 Aug 23;1238(1):94-8. doi: 10.1016/0005-2736(95)00130-u.
The sequence of the gene encoding the A subunit of the vacuolar type ATPase from Giardia lamblia is reported. Comparison of the encoded protein with the homologous subunits of eukaryotic and archaebacterial ATPases reveals high levels of similarity throughout the sequence (e.g., overall 49.1 and 44.6% identity to the homologous subunit from carrot and Halobacterium, respectively). An exception are three regions which are unique to the Giardia subunit. The largest of these regions contains motifs characteristic for eukaryotic spliceosomal introns; however, comparison to the cDNA shows that this region is also present in the mRNA.
报道了来自蓝氏贾第鞭毛虫的液泡型ATP酶A亚基的编码基因序列。将编码的蛋白质与真核生物和古细菌ATP酶的同源亚基进行比较，结果显示整个序列具有高度相似性（例如，与胡萝卜和嗜盐菌的同源亚基的总体同一性分别为49.1%和44.6%）。有三个区域是贾第鞭毛虫亚基所特有的，属于例外情况。其中最大的区域包含真核生物剪接体内含子的特征基序；然而，与cDNA比较表明该区域在mRNA中也存在。
